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首页> 外文期刊>Journal of bacteriology >Nucleotide sequence and genetic characterization of staphylococcal bacteriophage L54a int and xis genes.
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Nucleotide sequence and genetic characterization of staphylococcal bacteriophage L54a int and xis genes.

机译：葡萄球菌噬菌体L54a int和xis基因的核苷酸序列和遗传特征。

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摘要

The nucleotide sequence of a staphylococcal bacteriophage L54a DNA fragment containing genes involved in site-specific recombination was determined. Mutations generated by in vitro mutagenesis were used to map and characterize the int and xis genes. The site-specific recombination functions are tightly clustered within a 1.75-kilobase stretch of DNA fragment with the gene order of attP-int-xis. The int and xis genes are transcribed divergently. The Int protein deduced from the nucleotide sequence has a molecular weight of 41,000. Int is a basic protein with 354 amino acids of which 72 are basic and 38 are acidic. The Xis protein consists of only 59 amino acids with a molecular weight of 7,180. Unlike the Xis proteins of the lambdoid bacteriophages which are all basic proteins, L54a Xis is an acidic protein containing 13 acidic and 8 basic amino acids. The Int protein is required in both integrative and excisive reactions, whereas Xis is only required in excisive reaction. A well-conserved 40-residue region, including three perfectly conserved residues found in 15 site-specific recombinases of the integrase family that have been characterized, was also found in the L54a Int protein.
机译：确定了包含与位点特异性重组有关的基因的葡萄球菌噬菌体L54a DNA片段的核苷酸序列。通过体外诱变产生的突变被用于定位和表征int和xis基因。位点特异性重组功能紧密结合在DNA片段的1.75千碱基延伸范围内，具有attP-int-xis的基因顺序。 int和xis基因的转录不同。由核苷酸序列推导的Int蛋白的分子量为41,000。 Int是具有354个氨基酸的碱性蛋白质，其中72个是碱性氨基酸，而38个是酸性氨基酸。 Xis蛋白仅包含59个氨基酸，分子量为7,180。与Lambdoid噬菌体的Xis蛋白都是基本蛋白不同，L54a Xis是一种酸性蛋白，包含13个酸性和8个碱性氨基酸。 Int蛋白在整合反应和兴奋反应中都需要，而Xis仅在兴奋反应中需要。在L54a Int蛋白中还发现了一个保守良好的40个残基区域，包括在整合酶家族的15个位点特异性重组酶中发现的三个完全保守的残基。

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