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首页> 外文期刊>Journal of bacteriology >Intracellular PPi concentration is not directly dependent on amount of inorganic pyrophosphatase in Escherichia coli K-12 cells.
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Intracellular PPi concentration is not directly dependent on amount of inorganic pyrophosphatase in Escherichia coli K-12 cells.

机译:细胞内PPi浓度并不直接取决于大肠杆菌K-12细胞中无机焦磷酸酶的量。

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摘要

No correlation was observed between the level of inorganic pyrophosphatase (PPase) and the intracellular concentration of PPi in Escherichia coli cells. In exponentially growing cells the intracellular PPi concentration was in every case 1.5 nmol/mg (dry weight) or about 0.5 mM, even though the amount of PPase was varied from 15 to 2,600% of the control amount by mutation or by using a multicopy plasmid with an inserted gene (ppa) encoding PPase. The PPi concentration could, however, be increased or decreased from the control level under some stressful conditions.
机译:在大肠杆菌细胞中,无机焦磷酸酶(PPase)的水平与PPi的细胞内浓度之间没有相关性。在指数增长的细胞中,即使通过突变或使用多拷贝质粒将PPase的量从对照量的15%变为2,600%,细胞内PPi的浓度在每种情况下均为1.5 nmol / mg(干重)或约0.5 mM具有插入的编码PPase的基因(ppa)。但是,在某些压力条件下,PPi浓度可能会比对照水平增加或降低。

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