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首页> 外文期刊>Journal of bacteriology >Isolation and molecular characterization of the ribosomal protein L6 homolog from Chlamydia trachomatis.
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Isolation and molecular characterization of the ribosomal protein L6 homolog from Chlamydia trachomatis.

机译：沙眼衣原体核糖体蛋白L6同源物的分离和分子表征。

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The cloning of a Chlamydia trachomatis eukaryotic cell-binding protein reported earlier from our laboratory (R. Kaul, K. L. Roy, and W. M. Wenman, J. Bacteriol. 169:5152-5156, 1987) represents an artifact generated by nonspecific recombination of chromosomal DNA fragments. However, the amino terminus of this plasmid-encoded fusion product demonstrated significant homology to Escherichia coli ribosomal protein L6. By using a 458-bp PstI-HindIII fragment of recombinant pCT161/18 (representing the 5' end of the cloned gene), we isolated and characterized a C. trachomatis homolog of the ribosomal protein L6 gene of E. coli. Sequence analysis of an 1,194-bp EcoRI-SacI fragment that encodes chlamydial L6 (designated CtaL6e) revealed a 552-bp open reading frame comprising 183 amino acids and encodes a protein with a molecular weight of 19,839. Interestingly, complete gene homology between C. trachomatis serovars L2 and J, each of which exists as a single copy per genome, was observed. Expression of a plasmid-encoded gene product is dependent on the lac promoter, since no product was obtained if the open reading frame was oriented in opposition to the lac promoter. Immunoblotting of purified ribosomes revealed functional, as well as antigenic, homology between the E. coli and C. trachomatis ribosomal L6 proteins.
机译：我们实验室较早报道的沙眼衣原体真核细胞结合蛋白的克隆（R.Kaul，KL Roy和WM Wenman，J.Bacteriol.169：5152-5156，1987）代表了由染色体DNA的非特异性重组产生的假象碎片。但是，此质粒编码的融合产物的氨基末端表现出与大肠杆菌核糖体蛋白L6的显着同源性。通过使用重组pCT161 / 18的458-bp PstI-HindIII片段（代表克隆基因的5'端），我们分离并鉴定了大肠杆菌核糖体蛋白L6基因的沙眼衣原体同源物。对编码衣原体L6（命名为CtaL6e）的1,194 bp EcoRI-SacI片段进行序列分析，揭示了一个552 bp的开放阅读框，包含183个氨基酸，并编码分子量为19,839的蛋白质。有趣的是，观察到沙眼衣原体血清型L2和J之间完全基因同源，每个基因组以单个拷贝存在。质粒编码的基因产物的表达依赖于lac启动子，因为如果开放阅读框与lac启动子相反，则得不到产物。纯化的核糖体的免疫印迹显示大肠杆菌和沙眼衣原体核糖体L6蛋白之间具有功能性和抗原性同源性。

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