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首页> 外文期刊>Journal of bacteriology >Limits to the role of palindromy in deletion formation.
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Limits to the role of palindromy in deletion formation.

机译：回文在缺失形成中的作用受到限制。

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We tested the effect of palindromy on deletion formation. This involved a study of reversion of insertion mutations in the pBR322 amp gene at a site where deletions end either in 9-bp direct repeats or in adjoining 4-bp direct repeats. Inserts of palindromic DNAs ranging from 10 to more than 26 bp and related nonpalindromic DNAs were compared. The frequency of deletions (selected as Ampr revertants) was stimulated by palindromy only at lengths greater than 26 bp. The 4-bp direct repeats, one component of which is located in the palindromic insert, were used preferentially as deletion endpoints with palindromes of at least 18 bp but not of 16 or 10 bp. We interpret these results with a model of slippage during DNA replication. Because deletion frequency and deletion endpoint location depend differently on palindrome length, we propose that different factors commit a molecule to undergo deletion and determine exactly where deletion endpoints will be.
机译：我们测试了回文对缺失形成的影响。这项研究涉及到pBR322 amp基因在缺失终止于9 bp直接重复或相邻4 bp直接重复的位点上恢复插入突变的研究。比较了回文DNA插入片段（范围从10到大于26 bp）和相关的非回文DNA。回文仅在长度大于26 bp的情况下刺激缺失的频率（选择为Ampr回复体）。其中一部分位于回文插入物中的4 bp直接重复序列优先用作回文缺失至少为18 bp但不是16或10 bp的缺失终点。我们用DNA复制过程中的滑动模型来解释这些结果。因为删除频率和删除终点位置取决于回文长度，所以我们建议不同的因素使分子发生删除并准确确定删除终点在哪里。

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