...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >The cloned avirulence gene avrPto induces disease resistance in tomato cultivars containing the Pto resistance gene.
【24h】

The cloned avirulence gene avrPto induces disease resistance in tomato cultivars containing the Pto resistance gene.

机译：克隆的无毒力基因avrPto在含有Pto抗性基因的番茄品种中诱导抗病性。

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Resistance of tomato plants to the bacterial pathogen Pseudomonas syringae pv. tomato race 0 is controlled by the locus Pto. A bacterial avirulence gene was cloned by constructing a cosmid library from an avirulent P. syringae pv. tomato race, conjugating the recombinants into a strain of P. syringae pv. maculicola virulent on a tomato cultivar containing Pto, and screening for those clones that converted the normally virulent phenotype to avirulence. The cloned gene, designated avrPto, reduced multiplication of P. syringae pv. tomato transconjugants specifically on Pto tomato lines, as demonstrated by bacterial growth curve analyses. Analysis of F2 populations revealed cosegregation of resistance to P. syringae pv. tomato transconjugants carrying avrPto with resistance to P. syringae pv. tomato race 0. Surprisingly, mutation of avrPto in P. syringae pv. tomato race 0 does not eliminate the avirulent phenotype of race 0, suggesting that additional, as yet uncharacterized, avirulence genes and/or resistance genes may contribute to specificity in the avrPto-Pto interaction. Genetic analysis indicates that this resistance gene(s) would be tightly linked to Pto. Interestingly, P. syringae pv. glycinea transconjugants carrying avrPto elicit a typical hypersensitive resistant response in the soybean cultivar Centennial, suggesting conservation of Pto function between two crop plants, tomato and soybean.
机译：番茄植物对细菌病原体丁香假单胞菌PV的抗性。番茄小种0由位点Pto控制。通过从无毒力丁香假单胞菌pv构建粘粒文库，克隆出细菌无毒力基因。番茄小种，将重组体缀合到丁香假单胞菌pv。 Maculicola在含有Pto的番茄品种上具有强毒力，并筛选将正常毒力表型转化为无毒力的那些克隆。克隆的基因称为avrPto，可减少丁香假单胞菌pv的繁殖。如细菌生长曲线分析所证实的，番茄转导结合物特异于Pto番茄品系。对F2种群的分析显示对丁香假​​单胞菌pv的抗性共分离。携带avrPto的番茄转导结合体，对丁香假单胞菌pv具有抗性。番茄种族0。令人惊讶的是，丁香假单胞菌pv中avrPto突变。番茄第0种族没有消除第0种族的无毒表型，这表明尚无特征的其他无毒基因和/或抗性基因可能有助于avrPto-Pto相互作用的特异性。遗传分析表明，这种抗性基因将与Pto紧密相连。有趣的是，丁香假单胞菌PV。携带avrPto的甘氨酸转导结合体在大豆栽培品种百年纪念中引发典型的超敏抗性反应，表明在两种农作物（番茄和大豆）之间Pto的功能得以保留。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号