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首页> 外文期刊>Journal of bacteriology >Heat-induced accumulation and futile cycling of trehalose in Saccharomyces cerevisiae.
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Heat-induced accumulation and futile cycling of trehalose in Saccharomyces cerevisiae.

机译：酿酒酵母中海藻糖的热诱导积累和无效循环。

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摘要

Heat shock resulted in rapid accumulation of large amounts of trehalose in Saccharomyces cerevisiae. In cultures growing exponentially on glucose, the trehalose content of the cells increased from 0.01 to 1 g/g of protein within 1 h after the incubation temperature was shifted from 27 to 40 degrees C. When the temperature was readjusted to 27 degrees C, the accumulated trehalose was rapidly degraded. In parallel, the activity of the trehalose-phosphate synthase, the key enzyme of trehalose biosynthesis, increased about sixfold during the heat shock and declined to the normal level after readjustment of the temperature. Surprisingly, the activity of neutral trehalase, the key enzyme of trehalose degradation, also increased about threefold during the heat shock and remained almost constant during recovery of the cells at 27 degrees C. In pulse-labeling experiments with [14C]glucose, trehalose was found to be turned over rapidly in heat-shocked cells, indicating that both anabolic and catabolic enzymes of trehalose metabolism were active in vivo. Possible functions of the heat-induced accumulation of trehalose and its rapid turnover in an apparently futile cycle during heat shock are discussed.
机译：热激导致啤酒酵母中大量海藻糖迅速积累。在葡萄糖上以指数方式生长的培养物中，温育温度从27摄氏度变为40摄氏度后1小时内，细胞的海藻糖含量从0.01克/克增加至1克/克蛋白质。当温度重新调节至27摄氏度时，积累的海藻糖迅速降解。同时，海藻糖磷酸合成酶（海藻糖生物合成的关键酶）的活性在热激过程中增加了约六倍，而在温度调节后降至正常水平。出乎意料的是，中性海藻糖酶（海藻糖降解的关键酶）的活性在热激过程中也增加了约三倍，并在27℃恢复细胞期间几乎保持恒定。在用[14C]葡萄糖进行脉冲标记的实验中，海藻糖为发现在热激细胞中其被迅速翻转，表明海藻糖代谢的合成代谢和分解代谢酶在体内均具有活性。讨论了热激过程中海藻糖的热诱导积累及其在明显无效的循环中快速周转的可能功能。

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