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首页> 外文期刊>Journal of bacteriology >Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase.
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Nucleotide sequence of the Escherichia coli gene for lipid A disaccharide synthase.

机译：脂质A二糖合酶的大肠杆菌基因的核苷酸序列。

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The lpxB gene of Escherichia coli, believed to be the structural gene for lipid A disaccharide synthase, is located in the min 4 region of the chromosome. It is adjacent to and clockwise of the lpxA gene, which is thought to encode UDP-N-acetylglucosamine acyltransferase. Preliminary evidence suggests that lpxA and lpxB are cotranscribed in the clockwise direction and thus constitute part of a previously unknown operon (D. N. Crowell, M. S. Anderson, and C. R. H. Raetz, J. Bacteriol. 168:152-159, 1986). We now report the complete nucleotide sequence of a 1,522-base-pair PvuII-HincII fragment known to carry the lpxB gene. This sequence contained an open reading frame of 1,149 base pairs, in agreement with the predicted size, location, and orientation of lpxB. There was a second open reading frame 5' to, and in the same orientation as, lpxB that corresponded to lpxA. The ochre codon terminating lpxA was shown to overlap the methionine codon identified as the initiation codon for lpxB, suggesting that these genes are cotranscribed and translationally coupled. A third open reading frame was also shown to begin at the 3' end of lpxB with analogous overlap between the opal codon terminating lpxB and the methionine codon that putatively initiates translation downstream of lpxB in the clockwise direction. These results argue that at least three genes constitute a translationally coupled operon in the min 4 region of the E. coli chromosome. The accompanying paper by Tomasiewicz and McHenry (J. Bacteriol. 169:5735-5744, 1987) presents 4.35 kilobases of DNA sequence, beginning at the 3' end of lpxB, and argues that dnaE and several other open reading frames may be members of this operon.
机译：被认为是脂质A二糖合酶的结构基因的大肠杆菌的lpxB基因位于染色体的第4区。它与lpxA基因相邻并顺时针方向排列，该基因被认为编码UDP-N-乙酰氨基葡糖酰基转移酶。初步证据表明，lpxA和lpxB沿顺时针方向共转录，因此构成了以前未知的操纵子的一部分（D. N. Crowell，M。S. Anderson和C. R. H. Raetz，J。Bacteriol。168：152-159，1986）。现在，我们报告已知携带lpxB基因的1,522个碱基对的PvuII-HincII片段的完整核苷酸序列。该序列包含1,149个碱基对的开放阅读框，与lpxB的预测大小，位置和方向一致。在对应于lpxA的lpxB处有第二开放阅读框5'，并且在与lpxB相同的方向上。已显示出终止于lpxA的cher石密码子与被鉴定为lpxB的起始密码子的蛋氨酸密码子重叠，表明这些基因是共转录并翻译偶联的。还显示了第三个开放阅读框始于lpxB的3'末端，在终止于lpxB的蛋白石密码子和甲硫氨酸密码子之间类似的重叠，该蛋氨酸密码子假定沿顺时针方向起始lpxB下游的翻译。这些结果表明，至少三个基因在大肠杆菌染色体的最小4区构成翻译偶联的操纵子。 Tomasiewicz和McHenry（J. Bacteriol。169：5735-5744，1987）随附的论文介绍了从lpxB的3'末端开始的4.35 kb的DNA序列，并指出dnaE和其他几个开放阅读框可能是这个操纵子。

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