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首页> 外文期刊>Journal of bacteriology >Protein secretion in Pseudomonas aeruginosa: molecular cloning and characterization of the xcp-1 gene.
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Protein secretion in Pseudomonas aeruginosa: molecular cloning and characterization of the xcp-1 gene.

机译：铜绿假单胞菌中的蛋白质分泌：xcp-1基因的分子克隆和表征。

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Pleiotropic mutations (xcp) affecting the secretion of proteins in Pseudomonas aeruginosa have been previously characterized and mapped at three different loci on the chromosome. The xcp-1 gene, which is located at the 0-min region, was isolated from a genomic bank containing DNA from P. aeruginosa PAO1. The recombinant cosmid pLX25 complemented the xcp::Tn5-751 insertion mutation previously described. The xcp-1 gene was located on pLX25 by mapping the insertion point of transposon Tn5-751 and by deletion and subcloning analysis. The xcp-1 gene was expressed when transcription was initiated from a tac promoter, and a 26,000-dalton protein was identified in Escherichia coli minicells. The Xcp-1 protein was associated with the membrane fraction of E. coli. A 30-kilodalton outer membrane protein was also affected by the xcp::Tn5-751 mutation in P. aeruginosa. The possible correlation between Xcp-1 and this protein is discussed.
机译：以前已经鉴定了影响铜绿假单胞菌蛋白质分泌的多亲性突变（xcp），并将其定位在染色体上的三个不同基因座上。从包含铜绿假单胞菌PAO1的DNA的基因组库中分离了位于0分钟区域的xcp-1基因。重组粘粒pLX25补充了先前描述的xcp :: Tn5-751插入突变。通过定位转座子Tn5-751的插入点并通过缺失和亚克隆分析，将xcp-1基因定位在pLX25上。当从tac启动子开始转录时，表达了xcp-1基因，并在大肠杆菌小细胞中鉴定出26,000道尔顿的蛋白质。 Xcp-1蛋白与大肠杆菌的膜部分有关。铜绿假单胞菌的xcp :: Tn5-751突变也影响了30公斤的外膜蛋白。讨论了Xcp-1与该蛋白之间的可能相关性。

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