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首页> 外文期刊>Journal of bacteriology >A BlnI restriction map of the Salmonella typhimurium LT2 genome.
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A BlnI restriction map of the Salmonella typhimurium LT2 genome.

机译：鼠伤寒沙门氏菌LT2基因组的BlnI限制图。

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BlnI or AvrII (5'-CCTAGG) sites are very rare in the Salmonella typhimurium LT2 genome. BlnI was used to construct a physical map which was correlated with the genetic map by using three methods. First, Tn10 carries BlnI sites, and the extra restriction sites produced by 34 genetically mapped Tn10 insertions were physically mapped by using pulsed-field gel electrophoresis. Second, six genetically mapped Mud-P22 prophage insertions were used to assign BlnI fragments. Integration of Mud-P22 introduces 30 kb of DNA that can easily be detected by a "shift up" in all but the largest BlnI fragments. Finally, induced Mud-P22 insertions package more than 100 kb of genomic DNA adjacent to one side of the insertion. Some of the smaller BlnI fragments were localized by hybridization to a dot blot array of 52 lysates from induced Mud-P22 insertions. Of the 10 BlnI sites mapped, 6 probably occur in or near the 16S rRNA genes at about 55, 71, 83, 86, 88.5, and 89.5 min. There is one BlnI site in the 90-kb pSLT plasmid. Two additional BlnI fragments of about 7 and 4 kb have not been localized. The size of the genome was estimated as 4.78 Mb (+/- 0.1 Mb) excluding pSLT but including prophages Fels-1 and Fels-2. One BlnI fragment that maps between 55 and 59 min showed a 40-kb reduction in size in a strain cured of the approximately 40-kb Fels-2 prophage.
机译：在鼠伤寒沙门氏菌LT2基因组中，BlnI或AvrII（5'-CCTAGG）位点非常罕见。使用BlnI来构建通过使用三种方法与遗传图谱相关的物理图谱。首先，Tn10带有BlnI位点，并通过使用脉冲场凝胶电泳对34个基因定位的Tn10插入产生的额外限制性位点进行了物理定位。其次，使用六个基因定位的Mud-P22前噬菌体插入片段来分配BlnI片段。 Mud-P22的整合引入了30 kb的DNA，可以通过除最大的BlnI片段之外的所有片段的“上移”轻松检测到。最后，诱导的Mud-P22插入在插入的一侧附近包装了超过100 kb的基因组DNA。一些较小的BlnI片段通过与来自诱导的Mud-P22插入的52个裂解物的斑点印迹阵列杂交而定位。在所绘制的10个BlnI位点中，有6个可能出现在16S rRNA基因中或附近，大约在55、71、83、86、88.5和89.5分钟。 90kb pSLT质粒中有一个BlnI位点。约7和4 kb的另外两个BlnI片段尚未定位。不包括pSLT，但包括噬菌体Fels-1和Fels-2，基因组的大小估计为4.78 Mb（+/- 0.1 Mb）。映射在55至59分钟之间的一个BlnI片段显示，在治愈了约40kb Fels-2噬菌体的菌株中，其大小减少了40kb。

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