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首页> 外文期刊>Journal of bacteriology >Nucleotide and deduced amino acid sequences of the lacR, lacABCD, and lacFE genes encoding the repressor, tagatose 6-phosphate gene cluster, and sugar-specific phosphotransferase system components of the lactose operon of Streptococcus mutans.
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Nucleotide and deduced amino acid sequences of the lacR, lacABCD, and lacFE genes encoding the repressor, tagatose 6-phosphate gene cluster, and sugar-specific phosphotransferase system components of the lactose operon of Streptococcus mutans.

机译：lacR，lacABCD和lacFE基因的核苷酸序列和推导的氨基酸序列，它们编码变形链球菌乳糖操纵子的阻遏物，塔格糖6-磷酸基因簇和糖特异性磷酸转移酶系统组分。

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摘要

The complete nucleotide sequences of lacRABCDF and partial nucleotide sequence of lacE from the lactose operon of Streptococcus mutans are presented. Comparison of the streptococcal lac determinants with those of Staphylococcus aureus and Lactococcus lactis indicate exceptional protein and nucleotide identity. The deduced polypeptides also demonstrate significant, but lower, sequence similarity with the corresponding lactose proteins of Lactobacillus casei. Additionally, LacR has sequence homology with the repressor (DeoR) of the Escherichia coli deoxyribonucleotide operon, while LacC is similar to phosphokinases (FruK and PfkB) from E. coli. The primary translation products of the lacRABCDFE genes are polypeptides of 251 (M(r) 28,713), 142 (M(r) 15,610), 171 (M(r) 18,950), 310 (M(r) 33,368), 325 (M(r) 36,495), 104 (M(r) 11,401), and 123 (NH2-terminal) amino acids, respectively. As inferred from their direct homology to the staphylococcal lac genes, these determinants would encode the repressor of the streptococcal lactose operon (LacR), galactose-6-phosphate isomerase (LacA and LacB), tagatose-6-phosphate kinase (LacC), tagatose-1,6-bisphosphate aldolase (LacD), and the sugar-specific components enzyme III-lactose (LacF) and enzyme II-lactose (LacE) of the S. mutans phosphoenolpyruvate-dependent phosphotransferase system. The nucleotide sequence encompassing the S. mutans lac promoter appears to contain repeat elements analogous to those of S. aureus, suggesting that repression and catabolite repression of the lactose operons may be similar in these organisms.
机译：给出了来自变形链球菌的乳糖操纵子的lacRABCDF的完整核苷酸序列和lacE的部分核苷酸序列。链球菌lac决定簇与金黄色葡萄球菌和乳酸乳球菌的比较表明蛋白质和核苷酸具有非凡的一致性。推导的多肽还显示出与干酪乳杆菌的相应乳糖蛋白显着但较低的序列相似性。此外，LacR与大肠杆菌脱氧核糖核苷酸操纵子的阻遏物（DeoR）具有序列同源性，而LacC与大肠杆菌的磷酸激酶（FruK和PfkB）相似。 lacRABCDFE基因的主要翻译产物是251（M（r）28,713），142（M（r）15,610），171（M（r）18,950），310（M（r）33,368），325（M （r）36,495），104（M（r）11,401）和123（NH2-末端）氨基酸。从它们与葡萄球菌lac基因的直接同源性推断，这些决定簇将编码链球菌乳糖操纵子（LacR），半乳糖6-磷酸异构酶（LacA和LacB），塔格糖6-磷酸激酶（LacC），塔格糖的阻遏物-1，6-双磷酸醛缩醛酸酶（LacD）和变形链球菌磷酸烯醇丙酮酸依赖性磷酸转移酶系统的糖特异性成分酶III-乳糖（LacF）和酶II-乳糖（LacE）。包含变形链球菌lac启动子的核苷酸序列似乎含有与金黄色葡萄球菌相似的重复元件，这表明在这些生物中乳糖操纵子的阻遏和分解代谢物阻遏可能相似。

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