An unusual gene containing a dnaJ N-terminal box flanks the putative origin of replication of Mycoplasma genitalium.

C C Bailey; K F Bott

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An unusual gene containing a dnaJ N-terminal box flanks the putative origin of replication of Mycoplasma genitalium.

机译：含有dnaJ N末端框的不寻常基因位于生殖器支原体复制的推定来源的侧面。

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Origins of replication are known to be highly conserved among widely divergent microbial species, with the gene order in those regions being dnaA-dnaN-recF-gyrB. On the basis of sequence identities to entries in GenBank, the gene order of a 6-kb fragment of Mycoplasma genitalium DNA was determined to be dnaN-orf311-gyrB-gyrA-serS, which is structurally similar to the ancestral origin of replication. We have directly linked the dnaN gene to the M. genitalium dnaA gene by PCR amplification. However, we found a novel open reading frame, designated orf311, in place of an expected sequence encoding recF. Orf311 contains a DnaJ box motif at its N terminus, but it has no overall homology to any other protein or sequence in the database. We are unable to detect any recF homolog in M. genitalium by hybridization or during a random sequencing survey of the genome.

机译：众所周知，复制起点在差异很大的微生物物种中高度保守，这些区域中的基因顺序为dnaA-dnaN-recF-gyrB。根据GenBank中条目的序列同一性，确定生殖器支原体DNA的6kb片段的基因顺序为dnaN-orf311-gyrB-gyrA-serS，其结构与复制祖先相似。我们已经通过PCR扩增将dnaN基因直接连接到生殖器支原体dnaA基因。但是，我们发现了一个新的开放阅读框，命名为orf311，代替了编码recF的预期序列。 Orf311在其N末端包含一个DnaJ盒基序，但与数据库中的任何其他蛋白质或序列没有整体同源性。我们无法通过杂交或在基因组的随机测序调查中检测到生殖器支原体中的任何recF同源物。

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《Journal of bacteriology》 |1994年第18期|共6页
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C C Bailey; K F Bott;
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中图分类微生物学;
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6. An unusual gene containing a dnaJ N-terminal box flanks the putative origin of replication of Mycoplasma genitalium. [O] . C C Bailey, K F Bott 1994

机译：含有dnaJ N末端盒的不寻常基因位于生殖器支原体复制的推定来源的侧面。
7. An unusual gene containing a dnaJ N-terminal box flanks the putative origin of replication of Mycoplasma genitalium. [O] . Bailey, C C, Bott, K F 1994

机译：含有dnaJ N末端盒的不寻常基因位于生殖器支原体复制的推定来源的侧面。

An unusual gene containing a dnaJ N-terminal box flanks the putative origin of replication of Mycoplasma genitalium.

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