Physical map of the genome of Acholeplasma oculi ISM1499 and construction of a Tn4001 derivative for macrorestriction chromosomal mapping.

E Tigges; F C Minion

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Physical map of the genome of Acholeplasma oculi ISM1499 and construction of a Tn4001 derivative for macrorestriction chromosomal mapping.

机译：圆球菌ISM1499基因组的物理图谱和Tn4001衍生物的构建，用于宏观限制性染色体作图。

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A physical chromosomal map of Acholeplasma oculi ISM1499 was constructed by using field inversion gel electrophoresis. To assist in the ordering of the chromosomal fragments, a modified transposon, Tn4001.1064, was constructed. It was also used to rescue mycoplasmal chromosomal sequences adjacent to transposon insertion sites in a one-step cloning procedure. The total size of the A. oculi ISM1499 genome was estimated to be 1,633 kb. The restriction enzyme sites for ApaI, BssHII, EagI, and SmaI were positioned on the map along with several transposon insertion sites.

机译：利用场反向凝胶电泳技术构建了圆球菌ISM1499的物理染色体图。为了协助染色体片段的排序，构建了修饰的转座子Tn4001.1064。它也可以一步一步克隆到转座子插入位点附近的支原体染色体序列中。 A. oculi ISM1499基因组的总大小估计为1,633 kb。 ApaI，BssHII，EagI和SmaI的限制性酶切位点以及几个转座子插入位点都位于图谱上。

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《Journal of bacteriology》 |1994年第4期|共4页
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E Tigges; F C Minion;
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中图分类微生物学;
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6. Physical map of the genome of Acholeplasma oculi ISM1499 and construction of a Tn4001 derivative for macrorestriction chromosomal mapping. [O] . E Tigges, F C Minion 1994

机译：圆球菌ISM1499基因组的物理图谱和用于宏观限制性染色体作图的Tn4001衍生物的构建。
7. Physical map of the genome of Acholeplasma oculi ISM1499 and construction of a Tn4001 derivative for macrorestriction chromosomal mapping. [O] . Tigges, E, Minion, F C 1994

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Physical map of the genome of Acholeplasma oculi ISM1499 and construction of a Tn4001 derivative for macrorestriction chromosomal mapping.

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