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首页> 外文期刊>Journal of bacteriology >A third genetic locus required for the formation of heterocysts in Anabaena sp. strain PCC 7120.
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A third genetic locus required for the formation of heterocysts in Anabaena sp. strain PCC 7120.

机译：在鱼腥藻中形成异质囊所需的第三个遗传基因座。株PCC 7120。

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Mutagenesis of Anabaena sp. strain PCC 7120 with a derivative of transposon Tn5 led to the isolation of a mutant strain, P6, in which heterocysts are not formed (A. Ernst, T. Black, Y. Cai, J.-M. Panoff, D. N. Tiwari, and C. P. Wolk, J. Bacteriol. 174:6025-6032, 1992). Reconstruction of the transposon mutation of P6 in the wild-type strain reproduced the phenotype of the original mutant. Analysis by pulsed-field gel electrophoresis localized the transposition at ca. 3.44 Mb on the physical map of the chromosome of wild-type Anabaena sp. The transposon was situated within an open reading frame (ORF), which we denote hetP, whose wild-type form was cloned and also sequenced. The predicted HetP protein was not found to show significant sequence similarity to other proteins. The mutation in strain P6 could be complemented by a clone of a fragment of wild-type DNA that includes hetP and at least one additional ORF 3' from hetP, but not by a clone that includes hetP as its only ORF. The latter clone proved highly toxic. The phenotype of the P6 mutant may, therefore, be due to a polar effect of the insertion of the transposon. Filaments of strain P6 and of the wild-type strain, when bearing the complementing fragment on a pDU1-based plasmid, showed an increased frequency of clustered heterocysts compared with that of the wild-type strain.
机译：鱼腥藻的诱变带有转座子Tn5衍生物的PCC 7120菌株导致了突变株P6的分离，其中没有形成异胚囊（A.Ernst，T.Black，Y.Cai，J.-M.Panoff，DN Tiwari和CP Wolk，J.Bacteriol.174：6025-6032，1992）。野生型菌株中P6转座子突变的重建再现了原始突变体的表型。通过脉冲场凝胶电泳的分析将转座定位在约。野生型鱼腥藻的染色体物理图谱上的3.44 Mb。转座子位于开放阅读框（ORF）中，我们将其称为hetP，其野生型已被克隆并测序。未发现预测的HetP蛋白与其他蛋白具有显着的序列相似性。菌株P6中的突变可以由包含hetP和来自hetP的至少一个其他ORF 3'的野生型DNA片段的克隆来补充，而不是由包含hetP作为其唯一ORF的克隆来补充。后者被证明具有剧毒。因此，P6突变体的表型可能是由于转座子插入的极性作用所致。当在基于pDU1的质粒上带有互补片段时，菌株P6和野生型菌株的细丝与野生型菌株相比，簇状异型囊的频率增加。

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