Specific binding of the NikA protein to one arm of 17-base-pair inverted repeat sequences within the oriT region of plasmid R64.

N Furuya; T Komano

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Specific binding of the NikA protein to one arm of 17-base-pair inverted repeat sequences within the oriT region of plasmid R64.

机译：NikA蛋白与质粒R64 oriT区域内17个碱基对的反向重复序列的一条臂特异性结合。

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Products of the nikA and nikB genes of plasmid R64 have been shown to form a relaxation complex with R64 oriT DNA and to function together as an oriT-specific nickase. We purified the protein product of the nikA gene. The purified NikA protein bound specifically to the oriT region of R64 DNA. Gel retardation assays and DNase I footprinting analyses indicated that the NikA protein bound only to the right arm of 17-bp inverted repeat sequences; the right arm differed from the left arm by a single nucleotide. The binding site is proximal to the nick site and within the 44-bp oriT core sequence. Binding of the NikA protein induced DNA bending within the R64 oriT sequence.

机译：已显示质粒R64的nikA和nikB基因的产物与R64 oriT DNA形成松弛复合物，并一起作为oriT特异性切口酶起作用。我们纯化了nikA基因的蛋白质产物。纯化的NikA蛋白与R64 DNA的oriT区特异性结合。凝胶阻滞分析和DNase I足迹分析表明，NikA蛋白仅与17 bp反向重复序列的右臂结合;右臂与左臂的区别在于单个核苷酸。结合位点位于切口位点的近端，并在44 bp oriT核心序列内。 NikA蛋白的结合诱导R64 oriT序列内的DNA弯曲。

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《Journal of bacteriology》 |1995年第1期|共6页
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N Furuya; T Komano;
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1. Specific binding of the NikA protein to one arm of 17-base-pair inverted repeat sequences within the oriT region of plasmid R64. [J] . N Furuya, T Komano Journal of bacteriology . 1995,第1期

机译：NikA蛋白与质粒R64 oriT区域内17个碱基对的反向重复序列的一条臂特异性结合。
2. Initiation and Termination of DNA Transfer during Conjugation of IncI1 Plasmid R64: Roles of Two Sets of Inverted Repeat Sequences within oriT in Termination of R64 Transfer [J] . Nobuhisa Furuya, Teruya Komano Journal of bacteriology . 2000,第11期

机译：IncI1质粒R64偶联过程中DNA转移的起始和终止：oriT内两组反向重复序列在R64转移终止中的作用
3. Cloning and nucleotide sequence of the oriT region of the IncI1 plasmid R64. [J] . T Komano, A Toyoshima, K Morita, Journal of bacteriology . 1988,第9期

机译：IncI1质粒R64的oriT区域的克隆和核苷酸序列。
4. Conversion Of Helix-turn-helix Motif Sequence-specific DNA Binding Proteins Into Site-specific DNA Cleavage Agents [C] . Ebright R.H., Ebright Y.W., Pendergrast P.S., Engineering in Medicine and Biology Society, 1990., Proceedings of the Twelfth Annual International Conference of the IEEE . 1990

机译：螺旋转螺旋基序序列特定的DNA结合蛋白到位点特异性DNA裂解剂的转换。
5. Identification of Structural Signatures Within Transcription Factor Binding Sites and Their Flanking Regions in Saccharomyces cerevisiae Enhanced Through DeltaTRX Based Multiple Sequence Alignment. [D] . Schulze, Katharina. 2014

机译：通过基于DeltaTRX的多序列比对增强酿酒酵母中转录因子结合位点及其侧翼区域内的结构特征。
6. Specific binding of the NikA protein to one arm of 17-base-pair inverted repeat sequences within the oriT region of plasmid R64. [O] . N Furuya, T Komano 1995

机译：NikA蛋白与质粒R64 oriT区域内17个碱基对的反向重复序列的一条臂特异性结合。
7. Specific binding of the NikA protein to one arm of 17-base-pair inverted repeat sequences within the oriT region of plasmid R64. [O] . Furuya, N, Komano, T 1995

机译：NikA蛋白与质粒R64 oriT区域内17个碱基对的反向重复序列的一条臂特异性结合。

Specific binding of the NikA protein to one arm of 17-base-pair inverted repeat sequences within the oriT region of plasmid R64.

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