...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Transcriptional analysis of the 16s rRNA gene in Rickettsia prowazekii.
【24h】

Transcriptional analysis of the 16s rRNA gene in Rickettsia prowazekii.

机译：立克次体立克次体中16s rRNA基因的转录分析。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The control of rRNA synthesis in the etiological agent of epidemic typhus, Rickettsia prowazekii, a slowly growing obligate intracytoplasmic bacterium, was investigated. Transcription of the rickettsial 16S rRNA gene (rrs), of which there is only a single copy, was controlled by a single promoter region, and the site for the initiation of transcription (base A) was found 117 bp upstream of the rrs coding region for the mature product. The promoter region contained an Escherichia coli promoter-like sequence, TTGACA-N17-TATAAC, centered 139 bp upstream of the coding region for the mature product. To investigate whether transcription of the rickettsial rrs responds to amino acid starvation conditions, total RNA was isolated from R. prowazekii-infected mouse L929 cells with or without methionine starvation. The level of newly synthesized 16S rRNA precursors in R. prowazekii, as analyzed by ribonuclease protection assays, decreased significantly after methionine starvation for 6 h and then recovered within 12 h after the addition of methionine. The chemical half-lives of the 16S rRNA precursors in the methionine-starved rickettsiae did not differ significantly from those in the normal rickettsiae. These results suggest that R. prowazekii regulates transcription of the rrs in response to amino acid starvation conditions.
机译：研究了流行性斑疹伤寒立克次体（Rickettsia prowazekii）（一种缓慢生长的专性胞质内细菌）的病原体中rRNA合成的控制。仅单个拷贝的立克次体16S rRNA基因（rrs）的转录受单个启动子区域控制，转录起始位点（碱基A）位于rrs编码区上游117 bp为成熟的产品。该启动子区域包含大肠杆菌启动子样序列TTGACA-N17-TATAAC，其位于成熟产物编码区上游139 bp的中心。为了研究立克次体rrs的转录是否对氨基酸饥饿条件起反应，从有或没有甲硫氨酸饥饿的感染丙酸杆菌的小鼠L929细胞中分离了总RNA。通过核糖核酸酶保护试验分析，在Prowazekii中新合成的16S rRNA前体的水平在蛋氨酸饥饿6小时后显着下降，然后在添加蛋氨酸后12小时内恢复。在蛋氨酸饥饿的立克次体中，16S rRNA前体的化学半衰期与正常立克次体中的半衰期没有显着差异。这些结果表明，Prowazekii R.响应氨基酸饥饿条件调节rrs的转录。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号