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首页> 外文期刊>Journal of bacteriology >cobB function is required for catabolism of propionate in Salmonella typhimurium LT2: evidence for existence of a substitute function for CobB within the 1,2-propanediol utilization (pdu) operon.
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cobB function is required for catabolism of propionate in Salmonella typhimurium LT2: evidence for existence of a substitute function for CobB within the 1,2-propanediol utilization (pdu) operon.

机译：鼠伤寒沙门氏菌LT2中丙酸酯的分解代谢需要cobB功能：在1,2-丙二醇利用（pdu）操纵子中存在CobB替代功能的证据。

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摘要

The cobB function of Salmonella typhimurium LT2 was defined in vivo as an alternative activity for the nicotinic acid mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase enzyme (CobT), which is involved in the assembly of the nucleotide loop of cobalamin in this bacterium (J. R. Trzebiatowski, G. A. O'Toole, and J. C. Escalante-Semerena, J. Bacteriol. 176:3568-3575, 1994). In this paper we document that, independent of their inability to substitute for CobT function, cobB mutants are unable to use propionate as a carbon and energy source. A plasmid carrying only a wild-type copy of cobB complemented the cobalamin biosynthesis and propionate catabolism phenotypes of cobB mutants, indicating that a lack of CobB was responsible for both phenotypes. We demonstrate the existence of a function encoded by the 1,2-propanediol utilization (pdu) operon, which when induced by 1,2-propanediol compensated for the lack of CobB during propionate catabolism but failed to compensate for CobT in the assembly of the nucleotide loop of cobalamin in a cobB cobT double mutant.
机译：鼠伤寒沙门氏菌LT2的cobB功能在体内被定义为烟酸单核苷酸：5,6-二甲基苯并咪唑磷酸核糖基转移酶（CobT）的另一种活性，该酶参与了该细菌中钴胺素核苷酸环的组装（JR Trzebiatowski ，GA O'Toole，和JC Escalante-Semerena，J.Bacteriol.176：3568-3575，1994）。在本文中，我们证明，cobB突变体无法替代CobT功能，无法使用丙酸酯作为碳和能源。仅携带野生型cobB拷贝的质粒补充了cobBalmin生物合成和cobB突变体的丙酸分解代谢表型，表明缺乏CobB引起这两种表型。我们证明了由1,2-丙二醇利用（pdu）操纵子编码的功能的存在，当被1,2-丙二醇诱导时，该功能补偿了丙酸分解代谢过程中CobB的缺乏，但未能补偿组装过程中的CobT。 cobB cobT双突变体中钴胺素的核苷酸环。

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