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首页> 外文期刊>Journal of bacteriology >Genetic Determinants of Immunity and Integration of Temperate Myxococcus xanthus Phage Mx8
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Genetic Determinants of Immunity and Integration of Temperate Myxococcus xanthus Phage Mx8

机译：温带黄色粘球菌噬菌体Mx8免疫和整合的遗传决定因素。

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摘要

An 8.1-kb fragment of the temperate Myxococcus xanthusphage Mx8 genome, when cloned into a plasmid vector, permits site-specific integration of the plasmid and confers superinfection immunity. Sequence analysis of a 9.5-kb region of Mx8 DNA containing this fragment reveals 19 densely packed open reading frames, four of which have predicted products with known or suspected activities. The Mx8 imm gene, required for superinfection immunity, has a sequence similar to that of Arabidopsis thalianaG-box-binding factor 1. Mx8 makes a DNA adenine methylase, Mox, and integrase, Int, related to other methylases and integrases. Theint gene has two alternate translation initiation codons within the extensively overlapping uoi (upstream of int) gene. Comparison of the predicted product of the uoi gene with Salmonella phage P22 and Streptomycesplasmid Xis proteins shows that temperate phage excisionases may use variations of a helix-turn-helix motif to recognize specific DNA sequences.
机译：温带的粘液球菌噬菌体Mx8基因组的8.1 ​​kb片段，克隆到质粒载体中后，可以进行质粒的位点特异性整合并赋予超级感染免疫力。含有该片段的Mx8 DNA的9.5-kb区域的序列分析揭示了19个密集包装的开放阅读框，其中四个预测的产物具有已知或可疑的活性。超级感染免疫力所需的Mx8 imm 基因的序列与拟南芥 G-box结合因子1相似。Mx8产生DNA腺嘌呤甲基化酶Mox，和整合酶Int，与其他甲基化酶和整合酶有关。 int 基因在广泛重叠的 uoi （int上游）基因内具有两个交替的翻译起始密码子。比较 uoi 基因与沙门氏菌噬菌体P22和链霉菌质粒Xis蛋白的预测结果表明，温带噬菌体切除酶可能利用了螺旋的变异-turn-helix主题识别特定的DNA序列。

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