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首页> 外文期刊>Journal of bacteriology >std1, a Gene Involved in Glucose Transport in Schizosaccharomyces pombe
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std1, a Gene Involved in Glucose Transport in Schizosaccharomyces pombe

机译：std1，一个参与粟酒裂殖酵母糖转运的基因

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A wild-type strain, Sp972 h?, ofSchizosaccharomyces pombe was mutagenized with ethylmethanesulfonate (EMS), and 2-deoxyglucose (2-DOG)-resistant mutants were isolated. Out of 300 independent 2-DOG-resistant mutants, 2 failed to grow on glucose and fructose (mutants 3/8 and 3/23); however, their hexokinase activity was normal. They have been characterized as defective in their sugar transport properties, and the mutations have been designated as std1-8 andstd1-23 (sugar transport defective). The mutations are allelic and segregate as part of a single gene when the mutants carrying them are crossed to a wild-type strain. We confirmed the transport deficiency of these mutants by [14C]glucose uptake. They also fail to grow on other monosaccharides, such as fructose, mannose, and xylulose, as well as disaccharides, such as sucrose and maltose, unlike the wild-type strain. Lack of growth of the glucose transport-deficient mutants on maltose revealed the extracellular breakdown of maltose in S. pombe, unlike inSaccharomyces cerevisiae. Both of the mutants are unable to grow on low concentrations of glucose (10 to 20 mM), while one of them, 3/23, grows on high concentrations (50 to 100 mM) as if altered in its affinity for glucose. This mutant (3/23) shows a lag period of 12 to 18 h when grown on high concentrations of glucose. The lag disappears when the culture is transferred from the log phase of its growth on high concentrations. These mutants complement phenotypically similar sugar transport mutants (YGS4 and YGS5) reported earlier by Milbradt and Hoefer (Microbiology 140:2617–2623, 1994), and the clone complementing YGS4 and YGS5 was identified as the only glucose transporter in fission yeast having 12 transmembrane domains. These mutants also demonstrate two other defects: lack of induction and repression of shunt pathway enzymes and defective mating.
机译：用甲烷磺酸乙酯（EMS）诱变了粟酒裂殖酵母的野生菌株Sp972 h ？，并分离了耐2-脱氧葡萄糖（2-DOG）的突变体。在300个独立的2-DOG抗性突变体中，有2个不能在葡萄糖和果糖上生长（突变体3/8和3/23）;但是，它们的己糖激酶活性是正常的。它们的糖转运特性被表征为有缺陷，突变被命名为 std1-8 和 std1-23 （糖转运缺陷）。当携带突变的突变体与野生型菌株杂交时，这些突变是等位基因并作为单个基因的一部分分离。我们通过[ 14 C]葡萄糖摄取证实了这些突变体的运输缺陷。与野生型菌株不同，它们还不能在其他单糖（如果糖，甘露糖和木酮糖）以及二糖（如蔗糖和麦芽糖）上生长。麦芽糖上缺乏葡萄糖转运缺陷型突变体的生长表明 S中麦芽糖的细胞外分解。 pombe ，不同于 Saccharomyces cerevisiae 。两种突变体均无法在低浓度的葡萄糖（10至20 mM）上生长，而其中一个突变体3/23在高浓度的（50至100 mM）上生长，就好像其对葡萄糖的亲和力发生了变化。当在高浓度的葡萄糖上生长时，该突变体（3/23）显示出12至18小时的滞后时间。当培养物从高浓度生长的对数期转移时，滞后现象消失。这些突变体补充了表型相似的糖转运突变体（YGS4和YGS5），早于Milbradt和Hoefer（Microbiology 140：2617–2623，1994）报道，并且与YGS4和YGS5互补的克隆被认为是裂变酵母中唯一具有12个跨膜的葡萄糖转运蛋白。域。这些突变体还表现出另外两个缺陷：缺乏对分路酶的诱导和抑制以及有缺陷的交配。

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