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首页> 外文期刊>Journal of bacteriology >Initial Reactions in Anaerobic Oxidation ofm-Xylene by the Denitrifying Bacterium Azoarcussp. Strain T
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Initial Reactions in Anaerobic Oxidation ofm-Xylene by the Denitrifying Bacterium Azoarcussp. Strain T

机译：脱氮细菌偶氮菌在厌氧氧化间二甲苯中的初始反应。应变T

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摘要

The initial enzymatic steps in anaerobic m-xylene oxidation were studied in Azoarcus sp. strain T, a denitrifying bacterium capable of mineralizing m-xylene via 3-methylbenzoate. Permeabilized cells of m-xylene-grownAzoarcus sp. strain T catalyzed the addition ofm-xylene to fumarate to form (3-methylbenzyl)succinate. In the presence of succinyl coenzyme A (CoA) and nitrate, (3-methylbenzyl)succinate was oxidized toE-(3-methylphenyl)itaconate (or a closely related isomer) and 3-methylbenzoate. Kinetic studies conducted with permeabilized cells and whole-cell suspensions of m-xylene-grownAzoarcus sp. strain T demonstrated that the specific rate of in vitro (3-methylbenzyl)succinate formation accounts for at least 15% of the specific rate of in vivo m-xylene consumption. Based on these findings, we propose that Azoarcus sp. strain T anaerobically oxidizes m-xylene to 3-methylbenzoate (or its CoA thioester) via (3-methylbenzyl)succinate and E-(3-methylphenyl)itaconate (or its CoA thioester) in a series of reactions that are analogous to those recently proposed for anaerobic toluene oxidation to benzoyl-CoA. A deuterium kinetic isotope effect was observed in the (3-methylbenzyl)succinate synthase reaction (and the benzylsuccinate synthase reaction), suggesting that a rate-determining step in this novel fumarate addition reaction involves breaking a C-H bond.
机译：在 Azoarcus sp。中研究了厌氧 m -二甲苯氧化过程中的初始酶促步骤。菌株T，一种能够通过3-甲基苯甲酸使 m -二甲苯矿化的反硝化细菌。 m -二甲苯生长的 Azoarcus sp的透化细胞。菌株T催化了将 m 二甲苯加到富马酸酯中以形成（3-甲基苄基）琥珀酸酯。在琥珀酰辅酶A（CoA）和硝酸盐的存在下，（3-甲基苄基）琥珀酸酯被氧化为E-em-（3-甲基苯基）衣康酸酯（或密切相关的异构体）和3-甲基苯甲酸酯。用 m -二甲苯生长的 Azoarcus sp的透化细胞和全细胞悬液进行动力学研究。菌株T证明体外（3-甲基苄基）琥珀酸酯形成的比重至少占体内 m 二甲苯消耗比重的15％。根据这些发现，我们提出 Azoarcus sp。菌株T通过（3-甲基苄基）琥珀酸酯和 E -（3-甲基苯基）衣康酸酯（ m -二甲苯）将 m -二甲苯氧化为3-甲基苯甲酸酯（或其CoA硫酯） CoA硫酯），其反应类似于最近提出的将厌氧甲苯氧化为苯甲酰CoA的反应。在（3-甲基苄基）琥珀酸合酶反应（和苄基琥珀酸合酶反应）中观察到氘动力学同位素效应，表明该新型富马酸酯加成反应中的速率确定步骤涉及破坏C-H键。

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