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首页> 外文期刊>Journal of bacteriology >Functional Analysis of Glycosyltransferase Genes from Lactococcus lactis and Other Gram-Positive Cocci: Complementation, Expression, and Diversity
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Functional Analysis of Glycosyltransferase Genes from Lactococcus lactis and Other Gram-Positive Cocci: Complementation, Expression, and Diversity

机译：乳酸乳球菌和其他革兰氏阳性球菌的糖基转移酶基因的功能分析：互补，表达和多样性。

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Sixteen exopolysaccharide (EPS)-producing Lactococcus lactis strains were analyzed for the chemical compositions of their EPSs and the locations, sequences, and organization of theeps genes involved in EPS biosynthesis. This allowed the grouping of these strains into three major groups, representatives of which were studied in detail. Previously, we have characterized theeps gene cluster of strain NIZO B40 (group I) and determined the function of three of its glycosyltransferase (GTF) genes. Fragments of the eps gene clusters of strains NIZO B35 (group II) and NIZO B891 (group III) were cloned, and these encoded the NIZO B35 priming galactosyltransferase, the NIZO B891 priming glucosyltransferase, and the NIZO B891 galactosyltransferase involved in the second step of repeating-unit synthesis. The NIZO B40 priming glucosyltransferase gene epsD was replaced with an erythromycin resistance gene, and this resulted in loss of EPS production. This epsD deletion was complemented with priming GTF genes from gram-positive organisms with known function and substrate specificity. Although no EPS production was found with priming galactosyltransferase genes from L. lactis orStreptococcus thermophilus, complementation with priming glucosyltransferase genes involved in L. lactis EPS andStreptococcus pneumoniae capsule biosynthesis could completely restore or even increase EPS production in L. lactis.
机译：分析了16种产生外多糖（EPS）的乳酸乳球菌（emactococcus lactis）菌株的化学组成，以及参与EPS生物合成的 eps 基因的位置，序列和组织。这样就可以将这些菌株分为三大类，详细研究了其中的代表。以前，我们已经对NIZO B40菌株（第I组）的 eps 基因簇进行了表征，并确定了其三个糖基转移酶（GTF）基因的功能。克隆了菌株NIZO B35（II组）和NIZO B891（III组）的 eps 基因簇的片段，并编码了NIZO B35引发半乳糖基转移酶，NIZO B891引发葡萄糖基转移酶和NIZO B891半乳糖基转移酶参与重复单元合成的第二步。 NIZO B40引发葡萄糖基转移酶基因 epsD 被红霉素抗性基因取代，这导致EPS产生损失。这种 epsD 缺失与已知功能和底物特异性的革兰氏阳性生物的启动GTF基因互补。尽管未发现来自 L的启动半乳糖基转移酶基因的EPS产生。乳杆菌或嗜热链球菌，与涉及 L的引物葡糖基转移酶基因互补。乳酸 EPS和肺炎链球菌胶囊的生物合成可以完全恢复甚至提高 L中EPS的产量。乳酸。

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