...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Organization of the hao gene cluster of Nitrosomonas europaea: genes for two tetraheme c cytochromes.
【24h】

Organization of the hao gene cluster of Nitrosomonas europaea: genes for two tetraheme c cytochromes.

机译：欧洲亚硝化单胞菌hao基因簇的组织：两个四血红素c细胞色素的基因。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The organization of genes for three proteins involved in ammonia oxidation in Nitrosomonas europaea has been investigated. The amino acid sequence of the N-terminal region and four heme-containing peptides produced by proteolysis of the tetraheme cytochrome c554 of N. europaea were determined by Edman degradation. The gene (cycA) encoding this cytochrome is present in three copies per genome (H. McTavish, F. LaQuier, D. Arciero, M. Logan, G. Mundfrom, J.A. Fuchs, and A. B. Hooper, J. Bacteriol. 175:2445-2447, 1993). Three clones, representing at least two copies of cycA, were isolated and sequenced by the dideoxy-chain termination procedure. In both copies, the sequences of 211 amino acids derived from the gene sequence are identical and include all amino acids predicted by the proteolytic peptides. In two copies, the cycA open reading frame (ORF) is followed closely (three bases in one copy) by a second ORF predicted to encode a 28-kDa tetraheme c cytochrome not previously characterized but similar to the nirT gene product of Pseudomonas stutzeri. In one copy of the cycA gene cluster, the second ORF is absent.
机译：已经研究了欧洲亚硝基甲烷中涉及氨氧化的三种蛋白质的基因组织。通过Edman降解法确定了欧洲猪笼草的四血红素细胞色素c554的蛋白水解产生的N末端区域的氨基酸序列和四个含血红素的肽。每个基因组中存在编码该细胞色素的基因（cycA）3个拷贝（H. McTavish，F.LaQuier，D.Arciero，M.Logan，G.Mundfrom，JA Fuchs和AB Hooper，J.Bacteriol。175：2445 -2447，1993）。分离出代表cycA的至少两个拷贝的三个克隆，并通过双脱氧链终止方法测序。在两个拷贝中，源自基因序列的211个氨基酸的序列是相同的，并且包括蛋白水解肽预测的所有氨基酸。在两个拷贝中，cycA开放阅读框（ORF）紧跟着一个拷贝中的三个碱基（一个拷贝中有三个碱基），第二个ORF预计编码一种先前未表征但与假单胞菌的nirT基因产物相似的28 kDa四血红素c细胞色素。在cycA基因簇的一个副本中，没有第二个ORF。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号