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首页> 外文期刊>Journal of bacteriology >Transposon Tn5090 of plasmid R751, which carries an integron, is related to Tn7, Mu, and the retroelements.
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Transposon Tn5090 of plasmid R751, which carries an integron, is related to Tn7, Mu, and the retroelements.

机译：携带整合子的质粒R751的转座子Tn5090与Tn7，Mu和逆转录元件有关。

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Integrons confer on bacterial plasmids a capability of taking up antibiotic resistance genes by integrase-mediated recombination. We show here that integrons are situated on genetic elements flanked by 25-bp inverted repeats. The element carrying the integron of R751 has three segments conserved with similar elements in Tn21 and Tn5086. Several characteristics suggest that this element is a transposon, which we call Tn5090. Tn5090 was shown to contain an operon with three open reading frames, of which two, tniA and tniB, were predicted by amino acid similarity to code for transposition proteins. The product of tniA (559 amino acids) is a probable transposase with 25% amino acid sequence identity to TnsB from Tn7. Both of these polypeptides contain the D,D(35)E motif characteristic of a protein family made up of the retroviral and retrotransposon IN proteins and some bacterial transposases, such as those of Tn552 and of a range of insertion sequences. Like the transposase genes in Tn552, Mu, and Tn7, the tniA gene was followed by a gene, tniB, for a probable ATP-binding protein. The ends of Tn5090, like those of most other elements producing D,D(35)E proteins, begin by 5'-TG and also contains a complex structure with four 19-bp repeats at the left end and three at the right end. Similarly organized repeats have been observed earlier at the termini of both Tn7 and phage Mu, where they bind their respective transposases and have a role in holoenzyme assembly. Another open reading frame observed in Tn5090, tniC, codes for a recombinase of the invertase/resolvase family, suggesting a replicative transposition mechanism. The data presented here suggest that Tn5090, Tn7, Tn552, and Mu form a subfamily of bacterial transposons which in parallel to many insertion sequences are related to the retroelements.
机译：整合素赋予细菌质粒通过整合酶介导的重组吸收抗生素抗性基因的能力。我们在这里表明，整合素位于25 bp反向重复序列两侧的遗传元件上。携带R751整型的元件在Tn21和Tn5086中具有三个类似的保守区段。有几个特征表明该元件是转座子，我们称之为Tn5090。显示Tn5090包含一个带有三个开放阅读框的操纵子，其中两个tniA和tniB通过氨基酸相似性来预测转座蛋白的编码。 tniA的产物（559个氨基酸）是一种可能的转座酶，与来自Tn7的TnsB具有25％的氨基酸序列同一性。这两个多肽均包含由逆转录病毒和逆转座子IN蛋白以及某些细菌转座酶（例如Tn552的细菌转座酶和一系列插入序列）组成的蛋白质家族的D，D（35）E基序特征。像Tn552，Mu和Tn7中的转座酶基因一样，tniA基因后面紧跟一个可能是ATP结合蛋白的基因tniB。像大多数其他产生D，D（35）E蛋白的元件一样，Tn5090的末端以5'-TG开头，还包含一个复杂的结构，其左端有四个19-bp重复，右端有三个。在Tn7和噬菌体Mu的末端已观察到类似的有组织的重复序列，它们结合各自的转座酶并在全酶组装中起作用。在Tn5090中观察到的另一个开放阅读框tniC编码转化酶/分辨酶家族的重组酶，表明存在复制性转座机制。此处提供的数据表明Tn5090，Tn7，Tn552和Mu形成细菌转座子亚家族，其与许多插入序列平行都与反义元件有关。

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