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首页> 外文期刊>Journal of bacteriology >comK acts as an autoregulatory control switch in the signal transduction route to competence in Bacillus subtilis.
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comK acts as an autoregulatory control switch in the signal transduction route to competence in Bacillus subtilis.

机译：comK在枯草芽孢杆菌的信号转导途径中充当自动调节控制开关。

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The comK gene is a regulatory transcription unit which is essential for the development of genetic competence in Bacillus subtilis. The transcription of comK is under strict nutritional and growth phase-dependent control and has been shown to depend on the gene products of comA and srfA. In this report, we show that expression of comK is dependent on its own gene product as well as on the gene products of all other tested regulatory genes known to be involved in competence development (abrB, comA, comP, degU, sin, spo0A, spo0H, spo0K, and srfA). A mecA mutation is able to suppress the competence deficiency of mutations in any of these regulatory loci except for mutations in spo0A and, as we show here, in comK. Furthermore, we show that the presence of comK on a multiple copy plasmid leads to derepression of comK expression, causing an almost constitutive expression of competence in minimal medium as well as permitting competence development in complex medium. We infer from these results that the signals which trigger competence development, after having been received and processed by the various components of the competence signal transduction pathway, all converge at the level of comK expression. As soon as derepression of comK expression occurs, the positive autoregulation rapidly results in accumulation of the comK gene product, which subsequently induces competence.
机译：comK基因是一个调控转录单位，对于枯草芽孢杆菌的遗传能力的发展至关重要。 comK的转录受到严格的营养和生长阶段依赖性控制，并且已显示依赖于comA和srfA的基因产物。在本报告中，我们表明comK的表达取决于其自身的基因产物以及已知与能力发展有关的所有其他测试调节基因的基因产物（abrB，comA，comP，degU，sin，spo0A， spo0H，spo0K和srfA）。除了spo0A和comK中的突变外，mecA突变能够抑制任何这些调控基因座中突变的能力不足。此外，我们表明在多拷贝质粒上comK的存在导致comK表达的抑制，导致基本培养基中基本能力的组成表达以及在复杂培养基中的能力发展。我们从这些结果中推断，在已经由能力信号转导途径的各个组成部分接收并处理后，触发能力发展的信号全部收敛于comK表达水平。一旦出现comK表达抑制，阳性自动调节就会迅速导致comK基因产物积聚，从而诱导能力。

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