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首页> 外文期刊>Journal of bacteriology >spo0J is required for normal chromosome segregation as well as the initiation of sporulation in Bacillus subtilis.
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spo0J is required for normal chromosome segregation as well as the initiation of sporulation in Bacillus subtilis.

机译：spo0J是正常染色体分离以及枯草芽孢杆菌中孢子形成起始所必需的。

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摘要

The spo0J gene of Bacillus subtilis is required for the initiation of sporulation. We show that the sporulation defect caused by null mutations in spo0J is suppressed by a null mutation in the gene located directly upstream from spo0J, soj (suppressor of spo0J). These results indicate that Soj inhibits the initiation of sporulation and that Spo0J antagonizes that inhibition. Further genetic experiments indicated that Soj ultimately affects sporulation by inhibiting the activation (phosphorylation) of the developmental transcription factor encoded by spo0A. In addition, the temperature-sensitive sporulation phenotype caused by the ftsA279 (spoIIN279) mutation was partly suppressed by the soj null mutation, indicating that FtsA might also affect the activity of Soj. Soj and Spo0J are known to be similar in sequence to a family of proteins involved in plasmid partitioning, including ParA and ParB of prophage P1, SopA and SopB of F, and IncC and KorB of RK2, spo0J was found to be required for normal chromosome partitioning as well as for sporulation. spo0J null mutants produced a significant proportion of anucleate cells during vegetative growth. The dual functions of Spo0J could provide a mechanism for regulating the initiation of sporulation in response to activity of the chromosome partition machinery.
机译：枯草芽孢杆菌的spo0J基因是启动孢子形成所必需的。我们表明，由spo0J无效突变引起的孢子形成缺陷被位于spo0J，soj（spo0J的抑制子）直接上游的基因中的无效突变抑制。这些结果表明Soj抑制孢子形成的开始，而Spo0J拮抗该抑制作用。进一步的遗传实验表明，Soji通过抑制spo0A编码的发育转录因子的激活（磷酸化）而最终影响孢子形成。此外，由ftsA279（spoIIN279）突变引起的温度敏感性孢子表型被soj null突变部分抑制，表明FtsA也可能影响Soj的活性。已知Soj和Spo0J的序列与参与质粒分配的蛋白质家族相似，包括噬菌体P1的ParA和ParB，F的SopA和SopB以及RK2的IncC和KorB，发现spo0J是正常染色体所必需的分区以及孢子形成。 spo0J空突变体在营养生长过程中产生了大量的无核细胞。 Spo0J的双重功能可以提供一种机制，以响应染色体分配机制的活性来调节孢子形成的启动。

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