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首页> 外文期刊>Journal of bacteriology >Characterization of pilin genes from seven serologically defined prototype strains of Moraxella bovis.
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Characterization of pilin genes from seven serologically defined prototype strains of Moraxella bovis.

机译：表征从七个血清学定义的牛莫拉氏菌原型菌株的菌毛蛋白基因。

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Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characterized by nucleotide sequence determination. The coding sequences show extensive homology (70 to 80%) while the proximal downstream sequences show a dichotomy into nonhomologous sets. The pilin genes of three more strains were also characterized. The presence of an additional, partial pilin gene in each prototype strain was confirmed by Southern blot analysis, and the partial pilin genes from two strains of one serogroup were characterized by sequence determination. Features of the pilin gene sequences are considered in relation to pilin gene inversion and the serological variants of strains which may arise from gene inversion events.
机译：先前已通过血清学技术将牛莫拉氏菌的许多野外分离株分为七个血清群，每个血清群通过与针对单一原型菌株纯化菌毛制备的抗血清的同源交叉反应来定义。来自每个原型菌株的编码菌毛蛋白的基因已经通过核苷酸序列测定来表征。编码序列显示出广泛的同源性（70％到80％），而近端的下游序列显示出对非同源集的二分法。还表征了另外三个菌株的菌毛蛋白基因。通过Southern印迹分析确认每个原型菌株中存在另外的部分菌毛蛋白基因，并通过序列测定表征来自一个血清群的两个菌株的部分菌毛蛋白基因。考虑到菌毛蛋白基因序列的特征与菌毛蛋白基因倒置以及可能由基因倒置事件引起的菌株的血清学变异有关。

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