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首页> 外文期刊>Journal of bacteriology >Digital image analysis of growth and starvation responses of a surface-colonizing Acinetobacter sp.
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Digital image analysis of growth and starvation responses of a surface-colonizing Acinetobacter sp.

机译：数字图像分析的表面定殖不动杆菌的生长和饥饿反应。

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Surface growth of an Acinetobacter sp. cultivated under several nutrient regimens was examined by using continuous-flow slide culture, phase-contrast microscopy, scanning confocal laser microscopy, and computer image analysis. Irrigation of attached coccoid stationary-phase Acinetobacter sp. cells with high-nutrient medium resulted in a transition from coccoid to bacillar morphology. Digital image analysis revealed that this transition was biphasic. During phase I, both the length and the width of cells increased. In contrast, cell width remained constant during phase II, while both cell length and cell area increased at a rate greater than in phase I. Cells were capable of growth and division without morphological transition when irrigated with a low-nutrient medium. Rod-shaped cells reverted to cocci by reduction-division when irrigated with starvation medium. This resulted in conservation of cell area (biomass) with an increase in cell number. In addition, the changes in cell morphology were accompanied by changes in the stability of cell attachment. During phase I, coccoid cells remained firmly attached. Following transition in high-nutrient medium, bacillar cells displayed detachment, transient attachment, and drifting behaviors, resulting in a spreading colonization pattern. In contrast, cells irrigated with a low-nutrient medium remained firmly attached to the surface and eventually formed tightly packed microcolonies. It is hypothesized that the coccoid and bacillar Acinetobacter sp. morphotypes and associated behavior represent specialized physiological adaptations for attachment and colonization in low-nutrient systems (coccoid morphotype) or dispersion under high-nutrient conditions (bacillar morphotype).
机译：不动杆菌属的表面生长。通过使用连续流玻片培养，相差显微镜，扫描共聚焦激光显微镜和计算机图像分析来检查在几种营养方案下培养的番茄。附着的球状固定相不动杆菌属的灌溉。具有高营养培养基的细胞导致从球状形态转变为基底形态。数字图像分析表明，这种转变是双相的。在第一阶段，细胞的长度和宽度均增加。相反，在II期细胞宽度保持恒定，而细胞长度和细胞面积均以大于I期的速率增加。当用低营养培养基灌溉时，细胞能够生长和分裂而没有形态学转变。当用饥饿培养基灌溉时，杆状细胞通过还原分裂还原为球菌。随着细胞数量的增加，这导致细胞面积（生物量）的保存。另外，细胞形态的变化伴随着细胞附着稳定性的变化。在第一阶段，球状细胞保持牢固附着。在高营养培养基中过渡后，杆状细胞表现出分离，瞬时附着和漂移行为，从而导致定殖模式扩散。相反，用低营养培养基灌溉的细胞仍然牢固地附着在表面，并最终形成紧密堆积的微菌落。据推测，球状和杆状不动杆菌属。形态型和相关行为代表了针对低营养系统（球状形态型）或在高营养条件下分散（细菌形态型）的附着和定植的特殊生理适应。

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