...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >Genetic Studies of mrp, a Locus Essential for Cellular Aggregation and Sporulation of Myxococcus xanthus
【24h】

Genetic Studies of mrp, a Locus Essential for Cellular Aggregation and Sporulation of Myxococcus xanthus

机译：mrp的遗传学研究，这是黄色粘球菌细胞聚集和形成孢子必不可少的位点

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Under starvation conditions, Myxococcus xanthusundergoes a complex developmental process which includes cellular aggregation and sporulation. A transposon insertion mutant (the Tn5-Ω280 mutant) with defects in both aggregation and sporulation was analyzed in this study. The Tn5-Ω280 mutant was found to have a disrupted NtrC-like response regulator designated Myxococcusregulatory protein B (mrpB). Further sequencing analyses revealed a histidine kinase homolog (mrpA) immediately upstream of mrpB and a cyclic AMP receptor protein-like transcriptional regulator (mrpC) downstream ofmrpB. In-frame deletion analyses revealed that both themrpB and mrpC genes were required for cellular aggregation and sporulation but that only mrpAwas required for sporulation only. Site-specific mutagenesis of the putative phosphorylation site of MrpB, D58, showed that a D58A mutation caused defects in both aggregation and sporulation but that a D58E mutation resulted in only a sporulation defect. Further genetic and molecular analyses with reporter genes and reverse transcription-PCR indicated that mrpA and mrpB are cotranscribed but that mrpC is transcribed independently and that all of these genes are developmentally regulated. In addition, MrpB is essential for transcription of mrpC and MrpC regulates its own transcription. These data indicate that Mrp proteins are important components required for M. xanthusdevelopment. The complicated interaction between Mrp proteins may play an important role in regulating developmental gene expression inM. xanthus.
机译：在饥饿条件下，粘球菌经历了一个复杂的发育过程，其中包括细胞聚集和孢子形成。本研究分析了在聚集和孢子形成方面都存在缺陷的转座子插入突变体（Tn 5 -Ω280突变体）。发现Tn 5 -Ω280突变体具有破坏的NtrC样反应调节剂，称为粘球菌调节蛋白B（ mrpB ）。进一步的测序分析显示，紧接 mrpB 上游的组氨酸激酶同源物（ mrpA ）和下游的环状AMP受体蛋白样转录调节子（ mrpC ） mrpB 。帧内缺失分析显示， mrpB 和 mrpC 基因都需要细胞聚集和孢子形成，而只有 mrpA 只需要孢子形成。 MrpB的假定的磷酸化位点D58的位点特异性诱变表明，D58A突变引起聚集和孢子形成方面的缺陷，但D58E突变仅导致孢子形成缺陷。使用报告基因和逆转录PCR进行的进一步遗传和分子分析表明， mrpA 和 mrpB 是共转录的，但 mrpC 是独立转录的，所有这些基因中的一部分受到发育调控。此外，MrpB对于 mrpC 的转录至关重要，而MrpC调节其自身的转录。这些数据表明，Mrp蛋白是 M必需的重要成分。黄连开发。 Mrp蛋白之间复杂的相互作用可能在调控M发育基因表达中起重要作用。 xanthus 。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号