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首页> 外文期刊>Journal of bacteriology >Construction and Characterization of a cheA Mutant of Treponema denticola
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Construction and Characterization of a cheA Mutant of Treponema denticola

机译：密螺旋体cheA突变体的构建与表征

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The Treponema denticola cheA gene, encoding the central kinase of the general chemotaxis pathway, was analyzed for its role in chemotaxis and tissue penetration. The cheA gene was interrupted by insertion of an ermF-ermAM gene cassette. Reverse transcription-PCR confirmed that the other downstream chemotaxis genes within the same operon (cheW, cheX, and cheY) were still expressed in the cheA mutant strain. Lack of cheA resulted in decreased swarming on soft-agar swarm plates and failure to respond chemotactically to a mixture of nutrients. Behavioral analyses using video microscopy revealed that the cheA mutant exhibited coordinated cell movement. The cellular reversal frequency, however, was severely reduced, indicating that CheA in T. denticola mainly controls cellular reversal and that active chemotaxis signaling input is not required for coordination of flagellar rotation at both cell poles.
机译：分析了 Treponema denticola cheA 基因，该基因编码一般趋化途径的中心激酶，在趋化作用和组织渗透中的作用。 cheA 基因被插入一个 ermF-ermAM 基因盒而中断。逆转录-PCR证实同一操纵子内的其他下游趋化基因（ cheW ， cheX 和 cheY ）仍在< em> cheA 突变株。缺乏 cheA 导致软琼脂群上的蜂群减少，并且无法对营养混合物进行化学趋化反应。使用视频显微镜的行为分析表明， cheA 突变体表现出协调的细胞运动。然而，细胞逆转频率大大降低，表明在 T中存在CheA。 Denticola 主要控制细胞的逆转，并且不需要主动化学趋化信号输入来协调两个细胞极鞭毛的旋转。

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