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首页> 外文期刊>Journal of bacteriology >Salt-Dependent Expression of Glucosylglycerol-Phosphate Synthase, Involved in Osmolyte Synthesis in the Cyanobacterium Synechocystis sp. Strain PCC 6803
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Salt-Dependent Expression of Glucosylglycerol-Phosphate Synthase, Involved in Osmolyte Synthesis in the Cyanobacterium Synechocystis sp. Strain PCC 6803

机译：盐依赖性表达的葡萄糖基甘油磷酸合酶，参与蓝藻蓝藻属中的渗透压合成。应变PCC 6803

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The cyanobacterium Synechocystis sp. strain PCC 6803 is able to acclimate to levels of salinity ranging from freshwater to twice the seawater concentrations of salt by accumulating the compatible solute glucosylglycerol (GG). Expression of the ggpS gene coding for the key enzyme (glucosylglycerol-phosphate synthase) in GG synthesis was examined in detail. Under control conditions, the GgpS protein is stable, so that weak constitutive transcription of the ggpS gene resulted in a significant protein content. However, the enzyme activity was biochemically switched off, and no GG was detectable. After a salt shock, an immediate increase in mRNA content proportional to the salt content occurred, while the GgpS protein and GG contents rose in a linear manner. Furthermore, the stability of the ggpS mRNA increased transiently. In salt-acclimated cells expression of the ggpS gene, the GgpS protein content, and the amount of accumulated GG depended linearly on the external salt concentration. Mapping of the 5′ end of the ggpS transcript revealed a long nontranslated 5′ sequence and a putative typical cyanobacterial promoter, which did not show any obvious salt-regulatory element. The alternative σ factor σF was found to be involved in salt-dependent regulation of ggpS, since in a σF mutant induction of this gene was strongly reduced. The present study demonstrated that in addition to biochemical regulation of GgpS activity, alterations of ggpS expression are involved in regulation of GG synthesis in Synechocystis sp. strain PCC 6803. A model showing the interaction of the two regulatory levels is presented.
机译：蓝细菌 Synechocystis sp.。 PCC 6803菌株能够通过积累相容的溶质葡萄糖基甘油（GG）来适应盐度水平，从淡水到海水盐浓度的两倍。详细研究了GG合成中编码关键酶（葡萄糖基甘油磷酸合酶）的 ggpS 基因的表达。在控制条件下，GgpS蛋白是稳定的，因此 ggpS 基因的弱组成型转录导致大量蛋白质含量。然而，酶活性被生化关闭，并​​且未检测到GG。盐冲击后，与盐含量成正比的mRNA含量立即增加，而GgpS蛋白和GG含量呈线性增加。此外， ggpS mRNA的稳定性瞬时增加。在盐适应的细胞中， ggpS 基因的表达，GgpS蛋白含量和积累的GG量线性依赖于外部盐浓度。的 ggpS 转录物的5'末端的映射显示一个长的非翻译5'序列和推定的典型的蓝细菌启动子，没有显示任何明显的盐调节元件。发现替代σ因子σ F 参与了 ggpS 的盐依赖性调控，因为该基因的σ F 突变体诱导被大大减少。本研究表明，除了生化调节GgpS活性外， ggpS 表达的改变还参与了 Synechocystis sp的GG合成的调节。菌株PCC6803。提供了显示两个调节水平相互作用的模型。

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