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首页> 外文期刊>Journal of bacteriology >Gonococcal transferrin-binding protein 2 facilitates but is not essential for transferrin utilization.
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Gonococcal transferrin-binding protein 2 facilitates but is not essential for transferrin utilization.

机译：淋球菌转铁蛋白结合蛋白2促进转铁蛋白的利用，但并非必需。

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Pathogenic Neisseria species have been shown to scavenge iron from transferrin (Tf), although the mechanism is not yet fully understood. Two iron-repressible proteins that exhibit Tf-binding activity have been identified. This work describes the cloning and sequencing of tbpB, a 2.1-kb gene in N. gonorrhoeae that encodes Tbp2, an 85-kDa iron-repressible lipoprotein. Transcriptional interruption of tbpB had a strong polar effect on tbpA, the structural gene for Tbp1 that is located immediately downstream from tbpB. Such tbpB mutants did not express either Tbp2 or Tbp1, did not bind Tf to whole cells, did not grow on Tf plates, and did not take up iron from Tf. A mutant in which most of tbpB was deleted, presumably leaving tbpA under transcriptional control of the tbpB promoter, was constructed. This mutant did not express Tbp2 but expressed wild-type levels of Tbp1 and possessed the phenotype of reduced binding of Tf, decreased iron uptake from Tf, but normal growth on Tf plates. Mutants expressing Tbp2 and not Tbp1 bound less Tf, did not grow on Tf plates, and did not take up iron from Tf. These results suggest that tbpB and tbpA are polycistronic. Tbp2 apparently facilitates binding of Tf but is not essential for acquisition of iron from Tf under these in vitro conditions.
机译：致病性奈瑟氏菌物种已证明从转铁蛋白（Tf）中清除铁，尽管其机理尚未完全明了。已经鉴定出两种具有Tf结合活性的铁抑制蛋白。这项工作描述了tbpB的克隆和测序，tbpB是淋病奈瑟氏球菌中一个2.1 kb的基因，编码一个85 kDa的铁可抑制脂蛋白Tbp2。 tbpB的转录中断对tbpA具有很强的极性影响，tbpA是紧接tbpB下游的Tbp1的结构基因。这种tbpB突变体既不表达Tbp2也不表达Tbp1，不与Tf结合到整个细胞，也不在Tf板上生长，也不吸收Tf中的铁。构建了一个突变体，其中大部分的tbpB被删除，大概使tbpA处于tbpB启动子的转录控制之下。该突变体不表达Tbp2，但表达野生型水平的Tbp1，并具有减少Tf结合，减少从Tf吸收铁但在Tf平板上正常生长的表型。表达Tbp2而不表达Tbp1的突变体结合的Tf较小，在Tf平板上不生长，并且不吸收Tf中的铁。这些结果表明tbpB和tbpA是多顺反子。 Tbp2显然促进了Tf的结合，但对于在这些体外条件下从Tf中获取铁不是必需的。

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