Characterization of aarA, a pleiotrophic negative regulator of the 2'-N-acetyltransferase in Providencia stuartii.

P N Rather; E Orosz

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Characterization of aarA, a pleiotrophic negative regulator of the 2'-N-acetyltransferase in Providencia stuartii.

机译：aarA的特征，它是斯图亚特氏菌中2'-N-乙酰基转移酶的多营养负调节剂。

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We have utilized transposon mutagenesis to obtain insertional mutations in Providencia stuartii that activate the chromosomal aac(2')-la gene. Two closely linked mini-Tn5Cm insertions were obtained in a locus designated aarA, and a single insertion was obtained in a separate locus, aarC. Nucleotide sequence analysis, complementation studies, and localization of the sites of mini-Tn5Cm insertion have allowed the identification of the aarA coding region. The deduced AarA protein had a molecular mass of 31,086 kDa and displayed characteristics of an integral membrane protein. A strain deleted for the aarA gene by allelic exchange showed at least a fourfold increase in the accumulation of aac(2')-la mRNA and an eightfold increase in aminoglycoside resistance. Mutations in aarA were pleiotrophic and also resulted in loss of pigmentation and a deficiency in cell separation during division.

机译：我们已经利用转座子诱变来获得斯图亚特氏菌的插入突变，从而激活染色体aac（2'）-la基因。在命名为aarA的基因座中获得了两个紧密连接的mini-Tn5Cm插入，在单独的基因座aarC中获得了单个插入。核苷酸序列分析，互补研究和mini-Tn5Cm插入位点的定位已允许鉴定aarA编码区。推导的AarA蛋白的分子量为31,086 kDa，并显示出完整膜蛋白的特征。通过等位基因交换缺失了aarA基因的菌株显示aac（2'）-1a mRNA的积累至少增加了四倍，而氨基糖苷抗性增加了八倍。 aarA中的突变是多营养的，并且还导致色素沉着的丧失和分裂过程中细胞分离的缺乏。

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《Journal of bacteriology》 |1994年第16期|共5页
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P N Rather; E Orosz;
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中图分类微生物学;
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1. Overexpression and characterization of the chromosomal aminoglycoside 2'-N-acetyltransferase of Providencia stuartii. [J] . Franklin K, Clarke AJ Antimicrobial agents and chemotherapy. . 2001,第8期

机译：Providencia stuartii的染色体氨基糖苷2'-N-乙酰基转移酶的过表达和表征。
2. Characterization and transcriptional regulation of the 2'-N-acetyltransferase gene from Providencia stuartii. [J] . P N Rather, E Orosz, K J Shaw, Journal of bacteriology . 1993,第20期

机译：斯氏普罗维登斯（Provenncia stuartii）2'-N-乙酰基转移酶基因的表征和转录调控。
3. A regulatory cascade involving AarG, a putative sensor kinase, controls the expression of the 2'-N-acetyltransferase and an intrinsic multiple antibiotic resistance (Mar) response in Providencia stuartii. [J] . Rather PN, Paradise MR, Parojcic MM, Molecular Microbiology . 1998,第6期

机译：一个涉及AarG（一种假定的传感器激酶）的调控级联反应控制了Providencia stuartii中2'-N-乙酰基转移酶的表达和固有的多种抗生素抗性（Mar）反应。
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6. Characterization of aarA a pleiotrophic negative regulator of the 2-N-acetyltransferase in Providencia stuartii. [O] . P N Rather, E Orosz 1994

机译：aarA的特性它是斯图亚特氏菌中2-N-乙酰基转移酶的多营养负调节剂。
7. Characterization of aarA, a pleiotrophic negative regulator of the 2'-N-acetyltransferase in Providencia stuartii. [O] . Rather, P N, Orosz, E 1994

机译：aarA的特性，它是斯图亚特氏菌中2'-N-乙酰基转移酶的多营养负调节剂。

Characterization of aarA, a pleiotrophic negative regulator of the 2'-N-acetyltransferase in Providencia stuartii.

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