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首页> 外文期刊>Journal of bacteriology >Temperature affects the T-DNA transfer machinery of Agrobacterium tumefaciens.
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Temperature affects the T-DNA transfer machinery of Agrobacterium tumefaciens.

机译：温度影响根癌土壤杆菌的T-DNA转移机制。

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Early studies on Agrobacterium tumefaciens showed that development of tumors on plants following infection by A. tumefaciens was optimal at temperatures around 22 degrees C and did not occur at temperatures above 29 degrees C. To assess whether this inability to induce tumors is due to a defect in the T-DNA transfer machinery, mobilization of an incompatibility group Q (IncQ) plasmid by the T-DNA transfer machinery of A. tumefaciens was tested at various temperatures. Optimal transfer occurred when matings were performed at 19 degrees C, and transfer was not seen when matings were incubated above 28 degrees C. Transfer of the IncQ plasmid was dependent upon induction of the virB and virD operons by acetosyringone but was not dependent upon induction of the tra genes by octopine. However, alterations in the level of vir gene induction could not account for the decrease in transfer with increasing temperature. A. tumefaciens did successfully mobilize IncQ plasmids at higher temperatures when alternative transfer machineries were provided. Thus, the defect in transfer at high temperature is apparently in the T-DNA transfer machinery itself. As these data correlate with earlier tumorigenesis studies, we propose that tumor suppression at higher temperatures results from a T-DNA transfer machinery which does not function properly.
机译：根癌土壤杆菌的早期研究表明，被根癌土壤杆菌感染后，植物上肿瘤的发展在22摄氏度左右的温度下最佳，而在29摄氏度以上的温度下没有发生。要评估这种无法诱导肿瘤的原因是否是缺陷在T-DNA转移机制中，在不同温度下测试了根癌农杆菌的T-DNA转移机制对不相容基团Q（IncQ）质粒的动员。当在19°C进行交配时会发生最佳转移，而在28°C以上孵育时则看不到转移。IncQ质粒的转移取决于乙酰丁香酮对virB和virD操纵子的诱导，而不取决于对乙酰丁香酮的诱导。由章鱼碱的tra基因。但是，vir基因诱导水平的改变不能解释随着温度升高转移的减少。提供替代的转移机制后，根癌农杆菌确实成功地在较高温度下动员了IncQ质粒。因此，在高温下的转移缺陷显然是在T-DNA转移机制本身中。由于这些数据与早期的肿瘤发生研究相关，我们建议在较高温度下抑制肿瘤是由于T-DNA转移机制无法正常发挥作用。

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