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首页> 外文期刊>Journal of bacteriology >The GacS Sensor Kinase Regulates Alginate and Poly-β-Hydroxybutyrate Production in Azotobacter vinelandii
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The GacS Sensor Kinase Regulates Alginate and Poly-β-Hydroxybutyrate Production in Azotobacter vinelandii

机译：GacS传感器激酶调节葡萄固氮藻中藻酸盐和聚-β-羟基丁酸的产生

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摘要

Azotobacter vinelandii produces two polymers: the extracellular polysaccharide alginate and the intracellular polyester poly-β-hydroxybutyrate (PHB). A cosmid clone (pSMU588) from anA. vinelandii gene library diminished alginate production by A. vinelandii mucoid strain ATCC 9046. The nucleotide sequence and predicted amino acid sequence of the locus responsible for the mucoidy suppression revealed 65% identity toPseudomonas GacS, a transmembrane sensor kinase of the two-component regulators, whose cognate response regulator, GacA, is a global activator regulating several products and virulence factors. Plasmid pMC15, harboring gacS, and a strain carrying agacS nonpolar mutation were constructed. Either pMC15 or the gacS mutation significantly reduced alginate production and transcription of algD, the gene coding for the key enzyme GDP-mannose dehydrogenase of the alginate biosynthetic pathway. We found that the gacS mutation also reduced PHB accumulation and impaired encystment. Taken together, these data indicate that in A. vinelandii the gacSA global system regulates polymer synthesis.
机译：葡萄固氮菌产生两种聚合物：细胞外多糖藻酸盐和细胞内聚酯聚-β-羟基丁酸酯（PHB）。来自 A的粘粒克隆（pSMU588）。 vinelandii 基因库减少了 A产生的藻酸盐。 vinelandii 粘液样菌株ATCC9046。负责粘液抑制的基因座的核苷酸序列和预测的氨基酸序列显示与假单胞菌GacS（两组分的跨膜传感器激酶）具有65％的同一性。调节剂，其同源响应调节剂GacA，是调节多种产品和毒力因子的全球激活剂。构建了带有 gacS 的质粒pMC15和携带 gacS 非极性突变的菌株。 pMC15或 gacS 突变均可显着降低藻酸盐的产生和 algD 的转录，algD 是藻酸盐生物合成途径关键酶GDP-甘露糖脱氢酶的编码基因。我们发现 gacS 突变也减少了PHB的积累并损害了囊泡。这些数据加在一起表示 A。 vinelandii gacSA 全局系统调节聚合物的合成。

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