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首页> 外文期刊>Journal of bacteriology >Identification of an Operon Required for Ferrichrome Iron Utilization in Vibrio cholerae
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Identification of an Operon Required for Ferrichrome Iron Utilization in Vibrio cholerae

机译:霍乱弧菌中铁铬铁利用所需的操纵子的鉴定

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摘要

Mutagenesis of Vibrio cholerae with TnphoA, followed by screening for fusions that were activated under low-iron conditions, led to the identification of seven independent fusion strains, each of which was deficient in the ability to utilize ferrichrome as a sole iron source for growth in a plate bioassay and had an insertion in genes encoding products homologous toEscherichia coli FhuA or FhuD. Expression of the gene fusions was independent of IrgB but regulated by Fur. We report here a map of the operon and the predicted amino acid sequence of FhuA, based on the nucleotide sequence. Unlike those of the E. coli fhuoperon, the V. cholerae ferrichrome utilization genes are located adjacent and opposite in orientation to a gene encoding an ATP-binding cassette transporter homolog, but this gene, if disrupted, does not affect the utilization of ferrichrome in vitro.
机译:用Tn phoA 诱变霍乱弧菌,然后筛选在低铁条件下激活的融合物,从而鉴定出七个独立的融合菌株,每个菌株在平板生物测定法中缺乏利用铁铬酸作为唯一铁源进行生长的能力,并且在编码与大肠杆菌 FhuA或FhuD同源的产物的基因中插入。基因融合体的表达独立于IrgB,但受Fur调控。我们在此报告了基于核苷酸序列的操纵子图和FhuA的预测氨基酸序列。与 E不同。大肠杆菌Fhu 操纵子, V。霍乱弧菌的铬铁利用基因与编码ATP结合盒转运蛋白同源物的基因相邻且方向相反,但是如果该基因被破坏,则不会影响铬铁体外的利用。

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