...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of bacteriology >N-Acyl-Homoserine Lactone Inhibition of Rhizobial Growth Is Mediated by Two Quorum-Sensing Genes That Regulate Plasmid Transfer
【24h】

N-Acyl-Homoserine Lactone Inhibition of Rhizobial Growth Is Mediated by Two Quorum-Sensing Genes That Regulate Plasmid Transfer

机译：N-酰基-高丝氨酸内酯对根瘤菌生长的抑制作用由两个调控质粒转移的群体敏感基因介导。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The growth of some strains of Rhizobium leguminosarum bv. viciae is inhibited by N-(3-hydroxy-7-cis tetradecenoyl)-l-homoserine lactone (3OH-C14:1-HSL), which was previously known as the small bacteriocin before its characterization as an N-acyl homoserine lactone (AHL). Tn5-induced mutants of R. leguminosarum bv. viciae resistant to 3OH-C14:1-HSL were isolated, and mutations in two genes were identified. These genes, bisR and triR, which both encode LuxR-type regulators required for plasmid transfer, were found downstream of an operon containing trb genes involved in the transfer of the symbiotic plasmid pRL1JI. The first gene in this operon is traI, which encodes an AHL synthase, and the trbBCDEJKLFGHI genes were found between traI and bisR. Mutations in bisR, triR, traI, or trbL blocked plasmid transfer. Using gene fusions, it was demonstrated that bisR regulates triR in response to the presence of 3OH-C14:1-HSL. In turn, triR is then required for the induction of the traI-trb operon required for plasmid transfer. bisR also represses expression of cinI, which is chromosomally located and determines the level of production of 3OH-C14:1-HSL. The cloned bisR and triR genes conferred 3OH-C14:1-HSL sensitivity to strains of R. leguminosarum bv. viciae normally resistant to this AHL. Furthermore, bisR and triR made Agrobacterium tumefaciens sensitive to R. leguminosarum bv. viciae strains producing 3OH-C14:1-HSL. Analysis of patterns of growth inhibition using mutant strains and synthetic AHLs revealed that maximal growth inhibition required, in addition to 3OH-C14:1-HSL, the presence of other AHLs such as N-octanoyl-l-homoserine lactone and/or N-(3-oxo-octanoyl)-l-homoserine lactone. In an attempt to identify the causes of growth inhibition, a strain of R. leguminosarum bv. viciae carrying cloned bisR and triR was treated with an AHL extract containing 3OH-C14:1-HSL. N-terminal sequencing of induced proteins revealed one with significant similarity to the protein translation factor Ef-Ts.
机译：豆科根瘤菌 bv某些菌株的生长。蚕豆被 N -（3-hydroxy-7- cis 十四碳烯酰基）-1-高丝氨酸内酯（3OH-C 14：1 - HSL），以前称为 small 细菌素，后来被表征为 N -酰基高丝氨酸内酯（AHL）。 Tn 5 诱导的 R突变体。豆科植物 bv。分离到对3OH-C 14：1 -HSL具有抗性的蚕豆，并鉴定了两个基因的突变。这些基因 bisR 和 triR 均编码质粒转移所需的LuxR型调节子，位于含有 trb 基因的操纵子的下游。在共生质粒pRL1JI的转移中。该操纵子的第一个基因是 traI ，该基因编码AHL合酶，并且在 traI 和 bisR <之间找到了 trbBCDEJKLFGHI 基因。 / em>。 bisR ， triR ， traI 或 trbL 中的突变会阻止质粒转移。使用基因融合，证明 bisR 响应3OH-C 14：1 -HSL的存在而调节 triR 。然后，需要 triR 来诱导质粒转移所需的 traI-trb 操纵子。 bisR 还抑制 cinI 的表达，该蛋白位于染色体上并确定3OH-C 14：1 -HSL的产生水平。克隆的 bisR 和 triR 基因赋予3OH-C 14：1 -HSL对 R菌株敏感性。豆科植物 bv。蚕豆通常对此AHL具有抗性。此外， bisR 和 triR 使根癌农杆菌对 R敏感。豆科植物 bv。产生3OH-C 14：1 -HSL的蚕豆菌株。使用突变菌株和合成AHL对生长抑制模式的分析表明，除了3OH-C 14：1 -HSL以外，还需要最大的生长抑制，还需要其他AHL（例如 N < / em>-辛酰基-1-高丝氨酸内酯和/或 N -（3-氧代辛酰基）-1-高丝氨酸内酯。为了确定生长抑制的原因，使用了一种 R菌株。豆科植物 bv。用含有3OH-C 14：1 -HSL的AHL提取物处理带有克隆的 bisR 和 triR 的蚕豆。诱导蛋白的N端测序揭示了一种与蛋白翻译因子Ef-Ts极为相似的蛋白。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号