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首页> 外文期刊>Journal of bacteriology >Integration specificities of two lambdoid phages (21 and e14) that insert at the same attB site.
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Integration specificities of two lambdoid phages (21 and e14) that insert at the same attB site.

机译：在同一atB位置插入的两个lambdoid噬菌体（21和e14）的整合特异性。

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It was shown previously that phage 21 and the defective element e14 integrate at the same site within the icd gene of Escherichia coli K-12 but that 21 integrase and excisionase excise e14 in vivo very infrequently compared to excision of 21. We show here that the reverse is also true: e14 excises itself much better than it excises an adjacent 21 prophage. In vitro integrase assays with various attP substrates delimit the minimal attP site as somewhere between 366 and 418 bp, where the outer limits would include the outermost repeated dodecamers suggested as arm recognition sites by S. J. Schneider (Ph.D. dissertation, Stanford University, Stanford, Calif., 1992). We speculate that the reason 21 attP is larger than lambda attP (240 bp) is because it must include a 209-bp sequence homologous to the 3' end of the icd transcript in order to allow icd expression in lysogens. Alteration of portions of 21 attP to their e14 counterparts shows that 21 requires both the arm site and core site sequences of 21 but that replacements by e14 sequences function in some positions. Consistent with Schneider's in vivo results, and like all other known integrases from lambdoid phages, 21 requires integration host factor for activity.
机译：先前显示，噬菌体21和缺陷元件e14在大肠杆菌K-12的icd基因内的同一位点整合，但与21的切除相比，21整合和切除酶在体内却很少切除e14。反之亦然：e14切除自己的能力比切除相邻的21个噬菌体更好。具有各种atP底物的体外整合酶测定法将最小attP位点限定在366至418 bp之间，其中外部限制包括SJ Schneider建议作为手臂识别位点的最外面重复的十二聚体（斯坦福大学，斯坦福大学博士学位论文） ，加利福尼亚州，1992）。我们推测21 attP大于lambda attP（240 bp）的原因是，它必须包含与icd转录本3'端同源的209-bp序列，以便允许icd在溶原菌中表达。 21 attP的部分与其e14对应物的改变表明21需要a21的臂位和核心位点序列，但e14序列的替换在某些位置起作用。与Schneider的体内结果一致，并且像来自lambdoid噬菌体的所有其他已知整合一样，21需要整合宿主因子才能发挥活性。

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