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首页> 外文期刊>Journal of bacteriology >SacY, a Transcriptional Antiterminator fromBacillus subtilis, Is Regulated by Phosphorylation In Vivo
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SacY, a Transcriptional Antiterminator fromBacillus subtilis, Is Regulated by Phosphorylation In Vivo

机译：SacY，枯草芽孢杆菌的转录抗终止剂，通过体内磷酸化来调节。

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摘要

SacY antiterminates transcription of the sacB gene inBacillus subtilis in response to the presence of sucrose in the growth medium. We have found that it can substitute for BglG, a homologous protein, in antiterminating transcription of thebgl operon in Escherichia coli. We therefore sought to determine whether, similarly to BglG, SacY is regulated by reversible phosphorylation in response to the availability of the inducing sugar. We show here that two forms of SacY, phosphorylated and nonphosphorylated, exist in B. subtilis cells and that the ratio between them depends on the external level of sucrose. Addition of sucrose to the growth medium after SacY phosphorylation in the cell resulted in its rapid dephosphorylation. The extent of SacY phosphorylation was found to be proportional to the cellular levels of SacX, a putative sucrose permease which was previously shown to have a negative effect on SacY activity. Thus, the mechanism by which thesac sensory system modulates sacB expression in response to sucrose involves reversible phosphorylation of the regulator SacY, and this process appears to depend on the SacX sucrose sensor. The sac system is therefore a member of the novel family of sensory systems represented by bgl.
机译：响应于生长培养基中蔗糖的存在，SacY可以终止枯草芽孢杆菌中 sacB 基因的转录。我们发现，它可以替代同源蛋白BglG来阻止大肠杆菌中 bgl 操纵子的转录。因此，我们寻求确定响应于诱导糖的可用性，与BglG相似，SacY是否受到可逆磷酸化的调节。我们在这里显示 B中存在两种形式的SacY，磷酸化和非磷酸化。枯草杆菌细胞，它们之间的比例取决于蔗糖的外部水平。在细胞中SacY磷酸化后，将蔗糖添加到生长培养基中导致其快速去磷酸化。发现SacY的磷酸化程度与SacX的细胞水平成正比，SacX是一种假定的蔗糖通透酶，先前已证明对SacY活性具有负面影响。因此， sac 感觉系统响应蔗糖调节 sacB 表达的机制涉及调节剂SacY的可逆磷酸化，而这一过程似乎取决于SacX蔗糖传感器。因此， sac 系统是由 bgl代表的新型感觉系统家族的成员。

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