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首页> 外文期刊>Journal of bacteriology >Dimerization between the Holin and Holin Inhibitor of Phage λ
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Dimerization between the Holin and Holin Inhibitor of Phage λ

机译：Holin和噬菌体λ的Holin抑制剂之间的二聚化

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摘要

Holins are integral membrane proteins that control the access of phage-encoded muralytic enzymes, or endolysins, to the cell wall by the sudden formation of an uncharacterized homo-oligomeric lesion, or hole, in the membrane, at a precisely defined time. The timing of λ-infected cell lysis depends solely on the 107 codon Sgene, which encodes two proteins, S105 and S107, which are the holin and holin inhibitor, respectively. Here we report the results of biochemical and genetic studies on the interaction between the holin and the holin inhibitor. A unique cysteine at position 51, in the middle of the second transmembrane domain, is shown to cause the formation of disulfide-linked dimers during detergent membrane extraction. Forced oxidation of membranes containing S molecules also results in the formation of covalently linked dimers. This technique is used to demonstrate efficient dimeric interactions between S105 and S107. These results, coupled with the previous finding that the timing of lysis depends on the excess of the amount of S105 over S107, suggest a model in which the inhibitor functions by titrating out the effector in a stoichiometric fashion. This provides a basis for understanding two evolutionary advantages provided by the inhibitor system, in which the production of the inhibitor not only causes a delay in the timing of lysis, allowing the assembly of more virions, but also increases effective hole formation after triggering.
机译：holins是不可或缺的膜蛋白，可通过在精确定义的时间在膜中突然形成未表征的同型低聚病灶或孔来控制噬菌体编码的muralytic酶或溶血素进入细胞壁。受λ感染的细胞裂解的时间仅取决于107个密码子 S 基因，该基因编码两个蛋白S105和S107，分别是holin和holin抑制剂。在这里，我们报告了有关holin和holin抑制剂之间相互作用的生化和遗传研究结果。显示在第二跨膜结构域中间的位置51处的独特半胱氨酸在去污剂膜提取期间引起二硫键连接的二聚体的形成。含有S分子的膜的强迫氧化也导致共价连接的二聚体的形成。该技术用于证明S105和S107之间有效的二聚体相互作用。这些结果，加上先前的发现，即裂解时间取决于S105的量超过S107的发现，提示了一种模型，其中抑制剂通过以化学计量的方式滴定效应子来发挥作用。这为理解抑制剂系统提供的两个进化优势提供了基础，其中抑制剂的产生不仅导致裂解时间的延迟，允许更多毒粒的组装，而且还增加了触发后的有效孔形成。

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