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首页> 外文期刊>Journal of bacteriology >SdeK Is Required for Early Fruiting Body Development in Myxococcus xanthus
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SdeK Is Required for Early Fruiting Body Development in Myxococcus xanthus

机译：SdeK是粘果球菌早期子实体发育所必需的。

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Myxococcus xanthus cells carrying the Ω4408 Tn5lac insertion at the sde locus show defects in fruiting body development and sporulation. Our analysis ofsde expression patterns showed that this locus is induced early in the developmental program (0 to 2 h) and that expression increases approximately fivefold after 12 h of development. Further studies showed that expression of sde is induced as growing cells enter stationary phase, suggesting that activation of thesde locus is not limited to the developmental process. Because the peak levels of sde expression in both ansde + and an sde mutant background were similar, we conclude that the sde locus is not autoregulated. Characterization of the sde locus by DNA sequence analysis indicated that the Ω4408 insertion occurred within the sdeK gene. Primer extension analyses localized the 5′ end of sde transcript to a guanine nucleotide 307 bp upstream of the proposed start for the SdeK coding sequence. The DNA sequence in the ?12 and ?24 regions upstream of the sdetranscriptional start site shows similarity to the ?54family of promoters. The results of complementation studies suggest that the defects in development and sporulation caused by the Ω4408 insertion are due to an inactivation of sdeK. The predicted amino acid sequence of SdeK was found to have similarity to the sequences of the histidine protein kinases of two-component regulatory systems. Based on our results, we propose that SdeK may be part of a signal transduction pathway required for the activation and propagation of the early developmental program.
机译：在 sde 位点带有Ω4408Tn 5lac 插入序列的粘球菌xemhus 细胞显示出子实体发育和孢子形成的缺陷。我们对 sde 表达模式的分析表明，该基因座在发育程序的早期（0到2小时）被诱导，并且在发育12小时后表达增加了大约五倍。进一步的研究表明， sde 的表达在生长细胞进入稳定期时被诱导，这表明 sde 基因座的激活不仅限于发育过程。因为 sde + 和 sde 突变体背景中 sde 表达的峰值水平相似，所以我们得出结论 sde 位置未自动调节。通过DNA序列分析对 sde 基因座的表征表明，Ω4408插入发生在 sdeK 基因内。引物延伸分析将 sde 转录本的5'端定位于拟议的SdeK编码序列起始位点上游307 bp的鸟嘌呤核苷酸。在 sde 转录起始位点上游的？12和？24区域的DNA序列显示与启动子的？ 54 家族相似。互补研究的结果表明，由Ω4408插入引起的发育和孢子形成缺陷是由于 sdeK 失活引起的。发现SdeK的预测氨基酸序列与两组分调节系统的组氨酸蛋白激酶的序列相似。根据我们的结果，我们建议SdeK可能是早期发育程序的激活和传播所需的信号转导途径的一部分。

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