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首页> 外文期刊>Journal of bacteriology >The mutK Gene of Vibrio cholerae: a New Gene Involved in DNA Mismatch Repair
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The mutK Gene of Vibrio cholerae: a New Gene Involved in DNA Mismatch Repair

机译：霍乱弧菌的mutK基因：涉及DNA错配修复的新基因

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A new gene, mutK, of Vibrio cholerae, encoding a 19-kDa protein which is involved in repairing mismatches in DNA via a presumably methyl-independent pathway, has been identified. The product of the mutK gene cloned in either high- or low-copy-number vectors can reduce the spontaneous mutation frequency of Escherichia coli mutS, mutL,mutU, and dam mutants. The spontaneous mutation frequency of a chromosomal mutK knockout mutant was almost identical to that of wild-type V. cholerae cells, indicating that when the methyl-directed mismatch repair is blocked, the repair potential of MutK becomes apparent. The complete nucleotide sequence of the mutK gene has been determined, and the deduced amino acid sequence showed three open reading frames (ORFs), of which the ORF3 represents the mutK gene product. ThemutK gene product has no significant homology with any of the proteins deposited in the EMBL data bank. ORF2, located upstream ofmutK, encodes a 14-kDa protein which has more than 70% homology with a hypothetical protein found only downstream of theE. coli vsr gene. ORF1, located farther upstream ofmutK, has more than 80% homology with a major cold shock protein found in several bacteria. Downstream of mutK, a partial ORF having 60% homology with an RNA methyltransferase has been identified. The mutK gene has recently been positioned in the ordered cloned DNA map of the genome of the V. choleraestrain from which the gene was isolated (10).
机译：已经鉴定出霍乱弧菌的一个新基因 mutK ，该基因编码一个19-kDa的蛋白质，该蛋白质可能通过不依赖甲基的途径参与修复DNA的错配。克隆到高拷贝数或低拷贝数载体中的 mutK 基因产物可以降低大肠杆菌mutS ， mutL ， mutU 和 dam 突变体。染色体 mutK 敲除突变体的自发突变频率与野生型 V几乎相同。霍乱细胞，表明当甲基导向的错配修复被阻断时，MutK的修复潜力变得明显。确定了 mutK 基因的完整核苷酸序列，推导的氨基酸序列显示了三个开放阅读框（ORF），其中ORF3代表 mutK 基因产物。 mutK 基因产物与EMBL数据库中存放的任何蛋白质均无明显同源性。位于 mutK 上游的ORF2编码14kDa蛋白，该蛋白与仅在 E下游发现的假定蛋白具有70％以上的同源性。大肠杆菌vsr 基因。 ORF1位于mutK 的更上游，与几种细菌中发现的主要冷激蛋白具有80％以上的同源性。在 mutK 的下游，已鉴定出与RNA甲基转移酶具有60％同源性的部分ORF。 mutK 基因最近被定位在 V基因组的有序克隆DNA图谱中。分离出该基因的霍乱菌株（10）。

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