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首页> 外文期刊>Journal of bacteriology >Membrane-Mimicking Entities Induce Structuring of the Two-Peptide Bacteriocins Plantaricin E/F and Plantaricin J/K
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Membrane-Mimicking Entities Induce Structuring of the Two-Peptide Bacteriocins Plantaricin E/F and Plantaricin J/K

机译：膜模仿实体诱导二肽细菌素Plantaricin E / F和Plantarcin J / K的结构

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Lactobacillus plantarum C11 produces plantaricin E/F (PlnE/F) and plantaricin J/K (PlnJ/K), two bacteriocins whose activity depends on the complementary action of two peptides (PlnE and PlnF; PlnJ and PlnK). Three of the individual Pln peptides possess some antimicrobial activity, but the highest bacteriocin activity is obtained by combining complementary peptides in about a one-to-one ratio. Circular dichroism was used to study the structure of the Pln peptides under various conditions. All four peptides were unstructured under aqueous conditions but adopted a partly alpha-helical structure in the presence of trifluoroethanol, micelles of dodecylphosphocholine, and negatively charged dioleoylphosphoglycerol (DOPG) liposomes. Far less structure was induced by zwitterionic dioleoylglycerophosphocholine liposomes, indicating that a net negative charge on the phospholipid bilayer is important for a structure-inducing interaction between (positively charged) Pln peptides and a membrane. The structuring of complementary peptides was considerably enhanced when both (PlnE and PlnF or PlnJ and PlnK) were added simultaneously to DOPG liposomes. Such additional structuring was not observed in experiments with trifluoroethanol or dodecylphosphocholine, indicating that the apparent structure-inducing interaction between complementary Pln peptides requires the presence of a phospholipid bilayer. The amino acid sequences of the Pln peptides are such that the alpha-helical structures adopted upon interaction with the membrane and each other are amphiphilic in nature, thus enabling membrane interactions.
机译：植物乳杆菌 C11产生了植物抑素E / F（PlnE / F）和植物抑素J / K（PlnJ / K），这两种细菌素的活性取决于两种肽（PlnE和PlnF; PlnJ和PlnK）。三种单独的Pln肽具有一定的抗菌活性，但最高的细菌素活性是通过以约一比一的比例组合互补肽而获得的。圆二色性用于研究各种条件下Pln肽的结构。所有四种肽在水性条件下均未结构化，但在三氟乙醇，十二烷基磷酸胆碱胶束和带负电的油酰磷酸甘油（DOPG）脂质体存在下，部分采用α-螺旋结构。两性离子的油酰甘油磷脂酰胆碱脂质体诱导的结构少得多，这表明磷脂双层上的净负电荷对于（带正电荷的）Pln肽和膜之间的结构诱导相互作用很重要。当同时将两种（PlnE和PlnF或PlnJ和PlnK）同时添加到DOPG脂质体中时，互补肽的结构会大大增强。在用三氟乙醇或十二烷基磷酸胆碱进行的实验中未观察到这种额外的结构，这表明互补的Pln肽之间明显的结构诱导相互作用需要磷脂双层的存在。 Pln肽的氨基酸序列使得与膜相互作用时所采用的α-螺旋结构彼此之间是两亲的，因此能够进行膜相互作用。

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