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首页> 外文期刊>Journal of bacteriology >Requirement for the Enzymes Acetoacetyl Coenzyme A Synthetase and Poly-3-Hydroxybutyrate (PHB) Synthase for Growth of Sinorhizobium meliloti on PHB Cycle Intermediates
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Requirement for the Enzymes Acetoacetyl Coenzyme A Synthetase and Poly-3-Hydroxybutyrate (PHB) Synthase for Growth of Sinorhizobium meliloti on PHB Cycle Intermediates

机译：酶乙酰乙酰辅酶A合成酶和聚-3-羟基丁酸（PHB）合酶对在PHB循环中间体上的苜蓿中华根瘤菌生长的需求

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摘要

We have identified two Sinorhizobium melilotichromosomal loci affecting the poly-3-hydroxybutyrate degradation pathway. One locus was identified as the gene acsA, encoding acetoacetyl coenzyme A (acetoacetyl-CoA) synthetase. Analysis of the acsA nucleotide sequence revealed that this gene encodes a putative protein with a molecular weight of 72,000 that shows similarity to acetyl-CoA synthetase in other organisms. Acetyl-CoA synthetase activity was not affected in cell extracts of glucose-grown acsA::Tn5 mutants; instead, acetoacetyl-CoA synthetase activity was drastically reduced. These findings suggest that acetoacetyl-CoA synthetase, rather than CoA transferase, activates acetoacetate to acetoacetyl-CoA in the S. meliloti poly-3-hydroxybutyrate cycle. The second locus was identified as phbC, encoding poly-3-hydroxybutyrate synthase, and was found to be required for synthesis of poly-3-hydroxybutyrate deposits.
机译：我们已经确定了两个染色体位点，它们影响了聚3-羟基丁酸酯的降解途径。一个位点被鉴定为基因 acsA ，编码乙酰乙酰辅酶A（乙酰乙酰辅酶A）合成酶。对 acsA 核苷酸序列的分析表明，该基因编码一种推测的蛋白质，分子量为72,000，与其他生物体中的乙酰辅酶A合成酶相似。葡萄糖生长的 acsA :: Tn 5 突变体的细胞提取物中乙酰辅酶A合成酶的活性没有受到影响。相反，乙酰乙酰辅酶A合成酶活性大大降低。这些发现表明，在 S中，乙酰乙酰辅酶A合成酶而不是CoA转移酶将乙酰乙酸激活为乙酰乙酰辅酶A。 meliloti 聚-3-羟基丁酸酯循环。第二个基因座被鉴定为 phbC ，编码聚-3-羟基丁酸酯合成酶，被发现是合成聚-3-羟基丁酸酯沉积物所必需的。

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