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首页> 外文期刊>Journal of bacteriology >Characterization of the DNA- and Metal-Binding Properties of Vibrio anguillarum Fur Reveals Conservation of a Structural Zn2+ Ion
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Characterization of the DNA- and Metal-Binding Properties of Vibrio anguillarum Fur Reveals Conservation of a Structural Zn2+ Ion

机译:鳗弧菌毛皮的DNA和金属结合特性的表征揭示了结构Zn2 +离子的保守性。

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摘要

The ferric uptake regulator, Fur, represses iron uptake and siderophore biosynthetic genes under iron-replete conditions. Here we report in vitro solution studies on Vibrio anguillarum Fur binding to the consensus 19-bp Escherichia coli iron box in the presence of several divalent metals. We found that V. anguillarum Fur binds the iron box in the presence of Mn2+, Co2+, Cd2+, and to a lesser extent Ni2+ but, unlike E. coli Fur, not in the presence of Zn2+. We also found that V. anguillarum Fur contains a structural zinc ion that is necessary yet alone is insufficient for DNA binding.
机译:铁吸收调节剂Fur在铁充足的条件下抑制铁的吸收和铁载体的生物合成基因。在这里,我们报告了在几种二价金属存在下,鳗弧菌 Fur与共有的19 bp 大肠杆菌铁盒结合的体外溶液研究。我们发现 V。在Mn 2 + ,Co 2 + ,Cd 2 + 存在时,Fur将铁盒绑定Ni 2 + 的程度较小,但与 E不同。大肠杆菌,但不存在Zn 2 + 。我们还发现 V。 Anguillarum Fur含有结构性锌离子,这是必需的,但仅此不足以结合DNA。

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