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首页> 外文期刊>Journal of bacteriology >A Phosphopantetheinyl Transferase Homolog Is Essential for Photorhabdus luminescens To Support Growth and Reproduction of the Entomopathogenic NematodeHeterorhabditis bacteriophora
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A Phosphopantetheinyl Transferase Homolog Is Essential for Photorhabdus luminescens To Support Growth and Reproduction of the Entomopathogenic NematodeHeterorhabditis bacteriophora

机译：磷酸泛肽基转移酶同源物对于光致发光线虫以支持病原性线虫的生长和繁殖是必不可少的。

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The bacterium Photorhabdus luminescens is a symbiont of the entomopathogenic nematode Heterorhabditis bacteriophora. The nematode requires the bacterium for infection of insect larvae and as a substrate for growth and reproduction. The nematodes do not grow and reproduce in insect hosts or on artificial media in the absence of viable P. luminescens cells. In an effort to identify bacterial factors that are required for nematode growth and reproduction, transposon-induced mutants of P. luminescens were screened for the loss of the ability to support growth and reproduction of H. bacteriophora nematodes. One mutant, NGR209, consistently failed to support nematode growth and reproduction. This mutant was also defective in the production of siderophore and antibiotic activities. The transposon was inserted into an open reading frame homologous to Escherichia coli EntD, a 4′-phosphopantetheinyl (Ppant) transferase, which is required for the biosynthesis of the catechol siderophore enterobactin. Ppant transferases catalyze the transfer of the Ppant moiety from coenzyme A to a holo-acyl, -aryl, or -peptidyl carrier protein(s) required for the biosynthesis of fatty acids, polyketides, or nonribosomal peptides. Possible roles of a Ppant transferase in the ability of P. luminescens to support nematode growth and reproduction are discussed.
机译：细菌 Photorhabdus luminescens 是昆虫病原线虫 Herorhabditis bacteriophora 的共生体。线虫需要细菌来感染昆虫的幼虫，并作为其生长和繁殖的基质。在没有可行的 P的情况下，线虫不会在昆虫宿主或人工培养基中生长和繁殖。发光细胞。为了确定线虫生长和繁殖所需的细菌因子，转座子诱导的 P突变体。对luminescens 进行了筛选，以证明其缺乏支持 H生长和繁殖的能力。细菌线虫。一个突变体NGR209始终未能支持线虫的生长和繁殖。该突变体在铁载体和抗生素活性的产生中也有缺陷。将转座子插入到与大肠杆菌 Entem EntD（4'-磷酸泛锡基（Ppant）转移酶）同源的开放阅读框中，该酶是儿茶酚铁载体肠杆菌素的生物合成所必需的。 Ppant转移酶催化Ppant部分从辅酶A转移到脂肪酸，聚酮化合物或非核糖体肽生物合成所需的全酰基，-芳基或-肽基载体蛋白。 Ppant转移酶在 P能力中的可能作用。讨论了支持线虫生长和繁殖的夜光。

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