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首页> 外文期刊>Journal of bacteriology >The Hook Gene (flgE) Is Expressed from theflgBCDEF Operon in Rhodobacter sphaeroides: Study of an flgE Mutant
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The Hook Gene (flgE) Is Expressed from theflgBCDEF Operon in Rhodobacter sphaeroides: Study of an flgE Mutant

机译：Hook基因（flgE）在球形红球菌中从flgBCDEF操纵子表达：flgE突变体的研究

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In this work we identified the flgE gene encoding the flagellar hook protein from Rhodobacter sphaeroides. Our results show that this gene is part of a flagellar cluster that includes the genes flgB, flgC, flgD, flgE, andflgF. Two different types of mutants in theflgE gene were isolated, and both showed a Fla? phenotype, indicating the functionality of this sequence. Complementation studies of these mutant strains suggest thatflgE is included in a single transcriptional unit that starts in flgB and ends in flgF. In agreement with this possibility, a specific transcript of approximately 3.5 kb was identified by Northern blot. This mRNA is large enough to represent the complete flgBCDEF operon. FlgE showed a relatively high proline content; in particular, a region of 12 amino acids near the N terminus, in which four prolines were identified. Cells expressing a mutant FlgE protein lacking this region showed abnormal swimming behavior, and their hooks were curved. These results suggest that this region is involved in the characteristic quaternary structure of the hook of R. sphaeroides and also imply that a straight hook, or perhaps the rigidity associated with this feature, is important for an efficient swimming behavior in this bacterium.
机译：在这项工作中，我们鉴定了 flgE 基因，该基因编码 sphaeroides 的鞭毛钩蛋白。我们的结果表明，该基因是鞭毛簇的一部分，鞭毛簇包括基因 flgB，flgC，flgD，flgE 和 flgF 。分离了 flgE 基因中的两种不同类型的突变体，均表现出Fla ？表型，表明该序列的功能。对这些突变株的补充研究表明， flgE 包含在一个以 flgB 开始并以 flgF 结束的转录单元中。与这种可能性一致，通过Northern印迹鉴定了大约3.5kb的特异性转录物。该mRNA足够大，可以代表完整的 flgBCDEF 操纵子。 FlgE显示出较高的脯氨酸含量;特别是在N末端附近有12个氨基酸的区域，其中鉴定出4个脯氨酸。表达缺少该区域的突变FlgE蛋白的细胞表现出异常的游泳行为，并且钩子弯曲。这些结果表明该区域参与了 R钩的特征四元结构。并暗示直钩或与此特征相关的刚度对于该细菌的有效游泳行为很重要。

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