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首页> 外文期刊>Journal of bacteriology >Identification and Characterization of a Vibrio cholerae Gene, mbaA, Involved in Maintenance of Biofilm Architecture
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Identification and Characterization of a Vibrio cholerae Gene, mbaA, Involved in Maintenance of Biofilm Architecture

机译：霍乱弧菌基因mbaA的鉴定和表征，涉及维持生物膜结构

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摘要

The formation of biofilms is thought to play a key role in the environmental survival of the marine bacterium Vibrio cholerae. Although the factors involved in V. cholerae attachment to abiotic surfaces have been extensively studied, relatively little is known about the mechanisms involved in the subsequent maturation of the biofilms. Here we report the identification of a novel gene, which we have named mbaA (for maintenance of biofilm architecture), that plays a role in the formation and maintenance of the highly organized three-dimensional architecture of V. cholerae El Tor biofilms. We demonstrate that although the absence of mbaA does not significantly affect the initial attachment of cells onto the surface, it leads to the formation of biofilms that lack the typical structure, including the pillars of cells separated by fluid-filled channels that are evident in mature wild-type biofilms. Microscopic analysis indicates that the absence of mbaA leads to an increase in the amount of extracellular matrix material in the biofilms. The predicted mbaA product is a member of a family of regulatory proteins, containing GGDEF and EAL domains, suggesting that MbaA regulates the synthesis of some component of the biofilm matrix.
机译：生物膜的形成被认为在海洋细菌霍乱弧菌的环境生存中起着关键作用。尽管因素涉及 V 。 霍乱与非生物表面的附着已得到广泛研究，对生物膜随后成熟所涉及的机制了解甚少。在这里，我们报告了一个新基因的鉴定，我们将其命名为 mbaA （用于维持生物膜结构），该基因在高度组织化的三维结构的形成和维持中发挥了作用> V 。 霍乱 El Tor生物膜。我们证明，尽管缺少 mbaA 并不会显着影响细胞在表面上的初始附着，但会导致形成缺乏典型结构的生物膜，包括被流体分离的细胞柱。填补了成熟的野生型生物膜中明显的通道。显微镜分析表明，缺少 mbaA 会导致生物膜中细胞外基质物质的数量增加。预测的 mbaA 产品是包含GGDEF和EAL结构域的调节蛋白家族的成员，这表明MbaA调节生物膜基质某些成分的合成。

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