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首页> 外文期刊>Journal of bacteriology >Growth Phase-Coupled Alterations in Cell Structure and Function of Escherichia coli
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Growth Phase-Coupled Alterations in Cell Structure and Function of Escherichia coli

机译：大肠杆菌细胞结构和功能的生长期耦合变化。

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Escherichia coli cultures can be fractionated into more than 20 cell populations, each having a different bouyant density and apparently representing a specific stage of cell differentiation from exponential growth to stationary phase (H. Makinoshima, A. Nishimura, and A. Ishihama, Mol. Microbiol. 43:269-279, 2002). The density increase was found to be impaired at an early step for a mutant E. coli with the disrupted rpoS gene, which encodes the RNA polymerase RpoS (sigma-S) for stationary-phase gene transcription. This finding suggests that RpoS is need for the entire process of cell density increase. In the absence of RpoF sigma factor, the flagella are not formed as observed by electron microscopy, but the growth phase-coupled density increase takes place as in wild-type E. coli, confirming that the alteration in cell density is not directly correlated with the presence or absence of flagella. In the stationary-phase cells, accumulation of electron-dense areas was observed by electron microscopic observation of bacterial thin sections. By chemical determination, the increase in glycogen (or polysaccharides) was suggested to be one component, which contributes to the increase in weight-to-volume ratio of stationary-phase E. coli cells.
机译：大肠埃希氏菌培养物可分为20多个细胞群，每个细胞群具有不同的弹性强度，显然代表从指数生长期到固定期的细胞分化的特定阶段（H. Makinoshima，A。Nishimura，和A.Ishihama，Mol.Microbiol.43：269-279，2002）。发现突变体E的早期密度增加受到损害。 rpoS 基因被破坏的大肠杆菌，该基因编码用于固定相基因转录的RNA聚合酶RpoS（sigma-S）。这一发现表明，RpoS是细胞密度增加的整个过程所必需的。在不存在RpoF sigma因子的情况下，如通过电子显微镜观察到的那样，鞭毛不会形成，但与野生型Eem一样，会发生生长相耦合的密度增加。大肠杆菌，证实细胞密度的变化与鞭毛的存在与否没有直接关系。在固定相细胞中，通过电子显微镜观察细菌薄片来观察到电子致密区域的积累。通过化学测定，糖原（或多糖）的增加被认为是一种成分，这有助于增加固定相 E的重量体积比。大肠杆菌细胞。

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