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首页> 外文期刊>Journal of bacteriology >Genomic Sequence and Receptor for the Vibrio cholerae Phage KSF-1Φ: Evolutionary Divergence among Filamentous Vibriophages Mediating Lateral Gene Transfer
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Genomic Sequence and Receptor for the Vibrio cholerae Phage KSF-1Φ: Evolutionary Divergence among Filamentous Vibriophages Mediating Lateral Gene Transfer

机译：霍乱弧菌噬菌体KSF-1Φ的基因组序列和受体：介导侧向基因转移的丝状噬菌体之间的进化分歧。

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KSF-1Φ, a novel filamentous phage of Vibrio cholerae, supports morphogenesis of the RS1 satellite phage by heterologous DNA packaging and facilitates horizontal gene transfer. We analyzed the genomic sequence, morphology, and receptor for KSF-1Φ infection, as well as its phylogenetic relationships with other filamentous vibriophages. While strains carrying the mshA gene encoding mannose-sensitive hemagglutinin (MSHA) type IV pilus were susceptible to KSF-1Φ infection, naturally occurring MSHA-negative strains and an mshA deletion mutant were resistant. Furthermore, d-mannose as well as a monoclonal antibody against MSHA inhibited infection of MSHA-positive strains by the phage, suggesting that MSHA is the receptor for KSF-1Φ. The phage genome comprises 7,107 nucleotides, containing 14 open reading frames, 4 of which have predicted protein products homologous to those of other filamentous phages. Although the overall genetic organization of filamentous phages appears to be preserved in KSF-1Φ, the genomic sequence of the phage does not have a high level of identity with that of other filamentous phages and reveals a highly mosaic structure. Separate phylogenetic analysis of genomic sequences encoding putative replication proteins, receptor-binding proteins, and Zot-like proteins of 10 different filamentous vibriophages showed different results, suggesting that the evolution of these phages involved extensive horizontal exchange of genetic material. Filamentous phages which use type IV pili as receptors were found to belong to different branches. While one of these branches is represented by CTXΦ, which uses the toxin-coregulated pilus as its receptor, at least four evolutionarily diverged phages share a common receptor MSHA, and most of these phages mediate horizontal gene transfer. Since MSHA is present in a wide variety of V. cholerae strains and is presumed to express in the environment, diverse filamentous phages using this receptor are likely to contribute significantly to V. cholerae evolution.
机译：KSF-1Φ是霍乱弧菌的新型丝状噬菌体，可通过异源DNA包装支持RS1卫星噬菌体的形态发生，并促进水平基因转移。我们分析了KSF-1Φ感染的基因组序列，形态和受体，以及与其他丝状噬菌体的系统发育关系。带有编码 mshA 基因的甘露糖敏感性血凝素（MSHA）IV型菌毛的菌株易受KSF-1Φ感染，而天然存在的MSHA阴性菌株和 mshA 缺失突变体有抵抗力。此外，d-甘露糖以及抗MSHA的单克隆抗体抑制了噬菌体对MSHA阳性菌株的感染，这表明MSHA是KSF-1Φ的受体。噬菌体基因组包含7,107个核苷酸，包含14个开放阅读框，其中4个具有与其他丝状噬菌体同源的预测蛋白质产物。尽管丝状噬菌体的整体遗传组织似乎保留在KSF-1Φ中，但该噬菌体的基因组序列与其他丝状噬菌体的基因组序列没有高度同一性，并且显示出高度镶嵌的结构。分别对10种不同丝状噬菌体的假定复制蛋白，受体结合蛋白和Zot样蛋白进行编码的基因组序列的系统发育分析显示不同的结果，表明这些噬菌体的进化涉及遗传物质的广泛水平交换。发现使用IV型菌毛作为受体的丝状噬菌体属于不同的分支。虽然这些分支之一由CTXΦ代表，其使用毒素结合的菌毛作为其受体，但至少有四个进化分化的噬菌体共享一个共同的受体MSHA，并且这些噬菌体中的大多数介导水平基因转移。由于MSHA存在于各种各样的 V中。霍乱菌株，据推测可在环境中表达，使用该受体的各种丝状噬菌体可能对 V有重要贡献。霍乱的进化。

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