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首页> 外文期刊>Journal of bacteriology >Mutational analysis of a bacteriophage P4 late promoter.
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Mutational analysis of a bacteriophage P4 late promoter.

机译:噬菌体P4晚期启动子的突变分析。

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Transcription from the late Psid promoter of satellite bacteriophage P4 is dependent on the bacterial RNA polymerase carrying the sigma 70 subunit and is positively regulated by the product of the P4 delta gene or the ogr gene of helper bacteriophage P2. Through deletion and mutational analyses of the Psid promoter, we identified mutations in the -10 region and in a region of hyphenated dyad symmetry centered around position -55 that inactivate Psid. Most of these mutations alter base pairs that are highly conserved in the five other delta-activated P4 and P2 late promoters. We propose that the P4 delta and P2 ogr gene products bind the -55 region of the P4 and P2 late promoters.
机译:卫星噬菌体P4的晚期Psid启动子的转录取决于携带sigma 70亚基的细菌RNA聚合酶,并由P4δ基因或辅助噬菌体P2的ogr基因的产物正调控。通过删除和对Psid启动子的突变分析,我们确定了-10区和以-55位为中心的连字双对称区域中的突变使Psid失活。这些突变中的大多数会改变在其他五个激活的P4和P2晚期启动子中高度保守的碱基对。我们建议,P4 delta和P2 ogr基因产物结合P4和P2晚启动子的-55区。

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