Molecular cloning of an alcohol (butanol) dehydrogenase gene cluster from Clostridium acetobutylicum ATCC 824.

D J Petersen; R W Welch; F B Rudolph; G N Bennett

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Molecular cloning of an alcohol (butanol) dehydrogenase gene cluster from Clostridium acetobutylicum ATCC 824.

机译：丙酮丁醇梭菌ATCC 824中醇（丁醇）脱氢酶基因簇的分子克隆。

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In Clostridium acetobutylicum, conversion of butyraldehyde to butanol is enzymatically achieved by butanol dehydrogenase (BDH). A C. acetobutylicum gene that encodes this protein was identified by using an oligonucleotide designed on the basis of the N-terminal amino acid sequence of purified C. acetobutylicum NADH-dependent BDH. Enzyme assays of cell extracts of Escherichia coli harboring the clostridial gene demonstrated 15-fold-higher NADH-dependent BDH activity than untransformed E. coli, as well as an additional NADPH-dependent BDH activity. Kinetic, sequence, and isoelectric focusing analyses suggest that the cloned clostridial DNA contains two or more distinct C. acetobutylicum enzymes with BDH activity.

机译：在乙酰丁酸梭菌中，丁醛向丁醇的转化是通过丁醇脱氢酶（BDH）酶促实现的。通过使用基于纯化的丙酮丁醇梭菌NADH依赖性BDH的N-末端氨基酸序列设计的寡核苷酸，鉴定出编码该蛋白质的丙酮丁醇梭菌基因。带有梭菌基因的大肠杆菌细胞提取物的酶法分析显示，NADH依赖性BDH活性比未转化的大肠杆菌高15倍，另外还有NADPH依赖性BDH活性。动力学，序列和等电聚焦分析表明，克隆的梭菌DNA包含两个或多个具有BDH活性的丙酮丁醇梭菌酶。

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《Journal of bacteriology》 |1991年第5期|共4页
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D J Petersen; R W Welch; F B Rudolph; G N Bennett;
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1. Cloning, sequencing, and expression of clustered genes encoding beta-hydroxybutyryl-coenzyme A (CoA) dehydrogenase, crotonase, and butyryl-CoA dehydrogenase from Clostridium acetobutylicum ATCC 824. [J] . Z L Boynton, G N Bennet, F B Rudolph Journal of bacteriology . 1996,第11期

机译：丙酮丁醇梭菌ATCC 824中编码β-羟基丁酰辅酶A（CoA）脱氢酶，巴豆酶和丁酰CoA脱氢酶的簇基因的克隆，测序和表达。
2. Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824. [J] . R V Nair, G N Bennett, E T Papoutsakis Journal of bacteriology . 1994,第3期

机译：丙酮丁醇梭菌ATCC 824中醛/醇脱氢酶基因的分子特征
3. Molecular Characterization and Transcriptional Analysis of adhE2, the Gene Encoding the NADH-Dependent Aldehyde/Alcohol Dehydrogenase Responsible for Butanol Production in Alcohologenic Cultures of Clostridium acetobutylicum ATCC 824 [J] . Lisa Fontaine, Isabelle Meynial-Salles, Laurence Girbal, Journal of bacteriology . 2002,第3期

机译：adhE2的分子特征和转录分析，该基因编码NADH依赖的醛/醇脱氢酶，负责丙酮丁醇梭菌ATCC 824的产醇培养中的丁醇生产。
4. Development of Real-Time PCR Primer and Probe Sets for Detecting Degenerated and Non-degenerated Forms of the Butanol-Producing Bacterium Clostridium acetobutylicum ATCC 824 [C] . Sun-Mi Lee, Min Ok Cho, Youngsoon Um, Symposium on biotechnology for fuels and chemicals . 2010

机译：用于检测丁醇产物脱苯乙烯酸梭菌ATCC 824的丁醇产物菌梭菌梭菌的退化和非退化形式的实时PCR引物和探针组
5. Purification and studies of two butanol(ethanol) dehydrogenases and the effects of rifampicin and chloramphenicol on other enzymes important in the production of butyrate and butanol in Clostridium acetobutylicum ATCC 824. [D] . Welch, Richard William. 1990

机译：两种丁醇（乙醇）脱氢酶的纯化和研究以及利福平和氯霉素对乙酰丁酸梭菌ATCC 824中丁酸和丁醇生产中重要的其他酶的影响。
6. Molecular cloning of an alcohol (butanol) dehydrogenase gene cluster from Clostridium acetobutylicum ATCC 824. [O] . D J Petersen, R W Welch, F B Rudolph, 1991

机译：丙酮丁醇梭菌ATCC 824中醇（丁醇）脱氢酶基因簇的分子克隆。
7. Molecular cloning of an alcohol (butanol) dehydrogenase gene cluster from Clostridium acetobutylicum ATCC 824. [O] . Petersen, D J, Welch, R W, Rudolph, F B, 1991

机译：丙酮丁醇梭菌ATCC 824中醇（丁醇）脱氢酶基因簇的分子克隆。
8. Protoplast Formation, L-Colony Growth and Regeneration of Clostridium beijerinckii NRRL B-592 and B-593 and Clostridium acetobutylicum ATCC 10132. [R] . Birrer, G. A., Chesbro, W. R., Zsigray, R. M. 1989

机译：原生质体形成，L-菌落生长和拜氏梭菌NRRL B-592和B-593以及丙酮丁醇梭菌aTCC 10132的再生。

Molecular cloning of an alcohol (butanol) dehydrogenase gene cluster from Clostridium acetobutylicum ATCC 824.

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